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Microbiological evaluation of a new growth-based approach for rapid detection of methicillin-resistant Staphylococcus aureus
- Source :
- Journal of Antimicrobial Chemotherapy, 61(6), 1277-1280. Oxford University Press, Journal of Antimicrobial Chemotherapy
- Publication Year :
- 2008
-
Abstract
- OBJECTIVES: Recently, a rapid screening tool for methicillin-resistant Staphylococcus aureus (MRSA) has been introduced that applies a novel detection technology allowing the rapid presence or absence of MRSA to be determined from an enrichment broth after only a few hours of incubation. To evaluate the reliability of this new assay to successfully detect MRSA strains of different origin and clonality, well-characterized S. aureus strains were tested in this study.METHODS: More than 700 methicillin-susceptible and methicillin-resistant strains covering >90% of all registered European MRSA spa types within the SeqNet network were studied.RESULTS: All 513 MRSA strains tested were recognized as methicillin-resistant: among these, 96 MRSA strains were from an institutional collection, each presenting a unique spa type. None of the 211 methicillin-susceptible strains were detected as positive.CONCLUSIONS: The new growth-based rapid MRSA assay was shown to detect without exception all MRSA strains of large collections of strains comprising highly diverse genetic backgrounds, indicating that such a phenotypic test might be potentially more likely to cope with new strains.
- Subjects :
- Microbiology (medical)
Staphylococcus aureus
Micrococcaceae
Time Factors
Enrichment broth
Genotype
MRSA
Microbial Sensitivity Tests
medicine.disease_cause
Rapid detection
Sensitivity and Specificity
Microbiology
medicine
Humans
Pharmacology (medical)
Typing
Spa typing
Original Research
staphylococci
Pharmacology
biology
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
bacterial infections and mycoses
Methicillin-resistant Staphylococcus aureus
infection control
spa typing
Infectious Diseases
Methicillin Resistance
Subjects
Details
- Language :
- English
- ISSN :
- 03057453
- Database :
- OpenAIRE
- Journal :
- Journal of Antimicrobial Chemotherapy, 61(6), 1277-1280. Oxford University Press, Journal of Antimicrobial Chemotherapy
- Accession number :
- edsair.doi.dedup.....2ea998817b0f463de4a8f9752dcb22af