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Identification of Plet-1 as a specific marker of early thymic epithelial progenitor cells
- Source :
- Depreter, M G L, Blair, N F, Gaskell, T L, Nowell, C S, Davern, K, Pagliocca, A, Stenhouse, F H, Farley, A M, Fraser, A, Vrana, J, Robertson, K, Morahan, G, Tomlinson, S R & Blackburn, C C 2008, ' Identification of Plet-1 as a specific marker of early thymic epithelial progenitor cells ', Proceedings of the National Academy of Sciences, vol. 105, no. 3, pp. 961–966 . https://doi.org/10.1073/pnas.0711170105
- Publication Year :
- 2008
- Publisher :
- Proceedings of the National Academy of Sciences, 2008.
-
Abstract
- The thymus is essential for a functional immune system, because the thymic stroma uniquely supports T lymphocyte development. We have previously identified the epithelial progenitor population from which the thymus arises and demonstrated its ability to generate an organized functional thymus upon transplantation. These thymic epithelial progenitor cells (TEPC) are defined by surface determinants recognized by the mAbs MTS20 and MTS24, which were also recently shown to identify keratinocyte progenitor cells in the skin. However, the biochemical nature of the MTS20 and MTS24 determinants has remained unknown. Here we show, via expression profiling of fetal mouse TEPC and their differentiated progeny and subsequent analyses, that both MTS20 and MTS24 specifically bind an orphan protein of unknown function, Placenta-expressed transcript (Plet)-1. In the postgastrulation embryo, Plet-1 expression is highly restricted to the developing pharyngeal endoderm and mesonephros until day 11.5 of embryogenesis, consistent with the MTS20 and MTS24 staining pattern; both MTS20 and MTS24 specifically bind cell lines transfected with Plet-1 ; and antibodies to Plet-1 recapitulate MTS20/24 staining. In adult tissues, we demonstrate expression in a number of sites, including mammary and prostate epithelia and in the pancreas, where Plet-1 is specifically expressed by the major duct epithelium, providing a specific cell surface marker for this putative reservoir of pancreatic progenitor/stem cells. Plet-1 will thus provide an invaluable tool for genetic analysis of the lineage relationships and molecular mechanisms operating in the development, homeostasis, and injury in several organ/tissue systems.
- Subjects :
- Time Factors
Population
Thymus Gland
Pregnancy Proteins
Biology
Epithelium
Cell Line
Mice
medicine
Animals
Humans
RNA, Messenger
Progenitor cell
General
education
Progenitor
education.field_of_study
Membrane Glycoproteins
Multidisciplinary
Stem Cells
Mesonephros
Pancreatic Ducts
Gene Expression Regulation, Developmental
Epithelial Cells
Biological Sciences
Embryo, Mammalian
Molecular biology
Cell biology
Transplantation
medicine.anatomical_structure
Gene Expression Regulation
Cell culture
Antigens, Surface
Biological Markers
Endoderm
Stem cell
Biomarkers
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 105
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....2e8b536778f84906d758804d66bd50ac
- Full Text :
- https://doi.org/10.1073/pnas.0711170105