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Spontaneous development of a chromosomal translocation 5;14 in an epstein-barr-virus-associated b-cell lymphoma in aSCID mouse
- Source :
- International Journal of Cancer. 55:281-287
- Publication Year :
- 1993
- Publisher :
- Wiley, 1993.
-
Abstract
- C.B-17 SCID mice were inoculated with human peripheral blood leukocytes (PBLs) from normal Epstein-Barr virus (EBV)-seropositive and -seronegative donors. Confirmation of a functioning human immune response was demonstrated by the detection of human antibody after inoculation with rotavirus, tetanus toxoid, or EBV. One group of animals inoculated with PBLs from an EBV-seropositive donor developed immunoblastic lymphomas approximately 9 weeks after transplantation. Confirmation of the species and sex of origin of the tumor cells was established using a spontaneous cell line prepared from the tumor. At passage I, the tumor-cell line (AGTI) showed 15% of the metaphases with a translocation involving chromosomes 5 and 14. A lymphoblastoid cell line (AGLCL) established from the same PBLs from the same donor at the time of inoculation of the mice had a normal female karyotype. The AGLCL and a clone of AGTI cells were analyzed for rearrangement of immunoglobulin heavy chain (IgH) genes; both cell lines showed rearrangement of both IgH alleles. The results outlined in this report suggest that a spontaneous chromosomal translocation involving chromosome 14 occurred in normal PBLs in the SCID mouse.
- Subjects :
- Herpesvirus 4, Human
Cancer Research
Lymphoma, B-Cell
Gene Rearrangement, B-Lymphocyte, Heavy Chain
Clone (cell biology)
Chromosomal translocation
Mice, SCID
Antibodies, Viral
medicine.disease_cause
Translocation, Genetic
Virus
Mice
Tumor Cells, Cultured
medicine
Animals
Humans
B-cell lymphoma
Antigens, Viral
Chromosomes, Human, Pair 14
Genes, Immunoglobulin
biology
Gene rearrangement
medicine.disease
Epstein–Barr virus
Virology
DNA-Binding Proteins
Transplantation
Tumor Virus Infections
Epstein-Barr Virus Nuclear Antigens
Oncology
Immunoglobulin G
biology.protein
Chromosomes, Human, Pair 5
Female
Antibody
Neoplasm Transplantation
Subjects
Details
- ISSN :
- 10970215 and 00207136
- Volume :
- 55
- Database :
- OpenAIRE
- Journal :
- International Journal of Cancer
- Accession number :
- edsair.doi.dedup.....2e5e280d4d76772f3b3da4949914b23e