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Fibronectins containing extradomain A or B enhance osteoblast differentiation via distinct integrins
- Source :
- The Journal of biological chemistry. 292(19)
- Publication Year :
- 2016
-
Abstract
- Fibronectin is a multidomain protein secreted by various cell types. It forms a network of fibers within the extracellular matrix and impacts intracellular processes by binding to various molecules, primarily integrin receptors on the cells. Both the presence of several isoforms and the ability of the various domains and isoforms to bind to a variety of integrins result in a wide range of effects. In vivo findings suggest that fibronectin isoforms produced by the osteoblasts enhance their differentiation. Here we report that the isoform characterized by the presence of extradomain A activates α4β1 integrin and augments osteoblast differentiation. In addition, the isoform containing extradomain B enhances the binding of fibronectin through the RGD sequence to β3-containing integrin, resulting in increased mineralization by and differentiation of osteoblasts. Our study thus reveals novel functions for two fibronectin isoforms and the mediating receptors in osteoblast differentiation.
- Subjects :
- 0301 basic medicine
Gene isoform
DNA, Complementary
Alpha-v beta-3
Integrin
Green Fluorescent Proteins
Enzyme-Linked Immunosorbent Assay
Integrin alpha4beta1
Biochemistry
Extracellular matrix
03 medical and health sciences
chemistry.chemical_compound
Mice
Protein Domains
Osteogenesis
medicine
Cell Adhesion
Animals
Humans
Protein Isoforms
RNA, Small Interfering
Cell adhesion
Receptor
Molecular Biology
Osteoblasts
biology
Chemistry
Osteoblast
Cell Differentiation
3T3 Cells
Cell Biology
Integrin alphaVbeta3
Molecular biology
Cell biology
Extracellular Matrix
Fibronectins
Fibronectin
030104 developmental biology
medicine.anatomical_structure
Animals, Newborn
biology.protein
Oligopeptides
Protein Binding
Signal Transduction
Subjects
Details
- ISSN :
- 1083351X
- Volume :
- 292
- Issue :
- 19
- Database :
- OpenAIRE
- Journal :
- The Journal of biological chemistry
- Accession number :
- edsair.doi.dedup.....2e5673b4a3b811079a4348957c2ea25c