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Establishment of a new cell line from a patient with hairy cell leukemia-japanese variant
- Source :
- Leukemia & Lymphoma. 25:373-380
- Publication Year :
- 1997
- Publisher :
- Informa UK Limited, 1997.
-
Abstract
- A cell line, JHC-2, was established from the peripheral blood of a patient with hairy cell leukemia (HCL)-Japanese variant. The JHC-2 cells have cytologic features similar to those of the original tumor cells. They displayed hairy cytoplasmic projections by phase contrast and scanning electron microscopy. The tartrate-resistant acid phosphatase reaction was weakly positive. The immunophenotype of the JHC-2 cells was CD5-, CD10-, CD11c+/-, CD19+, CD21+, CD23+, CD24-, CD25+/-, CD38- and FMC-7+. The expression of surface immunoglobulin (IgG, kappa) and the configuration of Ig gene rearrangements in the JHC-2 cells were identical to those in the original leukemic cells, and the JHC-2 cells displayed trisomy 9 on cytogenetic examination. Southern blot analysis for the Epstein-Barr virus (EBV) genome showed that the JHC-2 cells contained the EBV genome, although the freshly isolated leukemic cells did not. These results indicate that the JHC-2 cell line is an EBV spontaneously transformed B cell line originating from HCL cells.
- Subjects :
- Male
Immunoglobulin gene
Herpesvirus 4, Human
Cancer Research
Acid Phosphatase
CD38
CD19
Immunoglobulin kappa-Chains
Immunophenotyping
Japan
Antigens, CD
hemic and lymphatic diseases
Tumor Cells, Cultured
medicine
Humans
Hairy cell leukemia
Gene Rearrangement
Leukemia, Hairy Cell
biology
Tartrate-Resistant Acid Phosphatase
Receptors, Interleukin-2
Hematology
Gene rearrangement
Middle Aged
medicine.disease
Virology
Molecular biology
Isoenzymes
Phenotype
Oncology
Cell culture
Immunoglobulin G
Antigens, Surface
biology.protein
CD5
Subjects
Details
- ISSN :
- 10292403 and 10428194
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Leukemia & Lymphoma
- Accession number :
- edsair.doi.dedup.....2e55ebeebacb4905a2a95b443d67edf9