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Effects of Basic Fibroblast Growth Factor on the Repair of Large Osteochondral Defects of Articular Cartilage in Rabbits: Dose–Response Effects and Long-Term Outcomes
Effects of Basic Fibroblast Growth Factor on the Repair of Large Osteochondral Defects of Articular Cartilage in Rabbits: Dose–Response Effects and Long-Term Outcomes
- Source :
- Tissue Engineering. 10:633-641
- Publication Year :
- 2004
- Publisher :
- Mary Ann Liebert Inc, 2004.
-
Abstract
- Articular cartilage possesses a limited capacity for self-renewal. The regenerated tissue often resembles fibrocartilage-like tissue rather than hyaline cartilage, and degeneration of the articular surface eventually occurs. The purpose of this study was to investigate the effect of basic fibroblast growth factor (bFGF) on the healing of full-thickness articular cartilage defects. bFGF (0, 10, 50, 100, 250, 500, or 1000 ng) was mixed with collagen gel and implanted into full-thickness articular cartilage defects drilled into rabbit knees. The repaired tissue was examined grossly and histologically, and was evaluated with the use of a grading scale at 4, 12, 24, and 50 weeks. At 4 weeks, treatment with 100 ng of bFGF had greatly stimulated cartilage repair both grossly and histologically in comparison with untreated defects (those filled with plain collagen gel). The average total scores on the histological grading scale were significantly better for the defects treated with bFGF than for the untreated defects. These improvements were evident as long as 50 weeks postoperatively, although slight deterioration was noted in the repaired cartilage. Immunohistochemical staining for type II collagen showed that this cartilage-specific collagen was diffusely distributed in the repaired tissue at 50 weeks. These findings suggest that bFGF may be a practical and important candidate for use in cartilage repair.
- Subjects :
- Pathology
medicine.medical_specialty
Time Factors
Basic fibroblast growth factor
Articular cartilage
Degeneration (medical)
chemistry.chemical_compound
Long term outcomes
medicine
Animals
Regeneration
Dose-Response Relationship, Drug
Hyaline cartilage
business.industry
General Engineering
Anatomy
Articular surface
Immunohistochemistry
Collagen gel
Cartilage
medicine.anatomical_structure
chemistry
Fibroblast Growth Factor 2
Rabbits
business
Grading scale
Subjects
Details
- ISSN :
- 15578690 and 10763279
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Tissue Engineering
- Accession number :
- edsair.doi.dedup.....2e45150b36a636805002386211b8142f
- Full Text :
- https://doi.org/10.1089/107632704323061988