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Whole-genome comparison of high and low virulent Staphylococcus aureus isolates inducing implant-associated bone infections
- Source :
- International journal of medical microbiology : IJMM. 308(5)
- Publication Year :
- 2017
-
Abstract
- Staphylococcus aureus can cause wide range of infections from simple soft skin infections to severe endocarditis, bacteremia, osteomyelitis and implant associated bone infections (IABI). The focus of the present investigation was to study virulence properties of S. aureus isolates from acute and chronic IABI by means of their in vivo lethality, in vitro osteoblasts invasion, biofilm formation and subsequently whole genome comparison between high and low virulent strains. Application of insect infection model Galleria mellonella revealed high, intermediate and low virulence phenotypes of these clinical isolates, which showed good correlation with osteoblast invasion and biofilm formation assays. Comparative genomics of selected high (EDCC 5458) and low (EDCC 5464) virulent strains enabled the identification of molecular factors responsible for the development of acute and chronic IABI. Accordingly, the low virulent strain EDCC 5464 harbored point mutations resulting in frame shift mutations in agrC (histidine kinase in agr system), graS (histidine kinase in graSR, a two component system) and efeB (peroxidase in efeOBU operon, an iron acquisition system) genes. Additionally, we found a mobile element (present 11 copies in EDCC 5464) inserted at the end of β-hemolysin (hlb) and sarU genes, which are involved in the pathogenesis and regulation of virulence gene expression in coordination with quorum sensing system. All these results are in good support with the low virulence behavior of EDCC 5464. From the previous literature, it is well known that agr defective S. aureus clinical strains are isolated from the chronic infections. Similarly, low virulent EDCC 5464 was isolated from chronic implant-associated bone infections infection whereas EDCC 5458 was obtained from acute implant-associated bone infections. Laboratory based in vitro and in vivo results and insights from comparative genomic analysis could be correlated with the clinical conclusion of IABIs and allows evidence-based treatment strategies based on the pathogenesis of the strain to cure life devastating implant-associated infections.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Staphylococcus aureus
Histidine Kinase
Virulence
Biology
Moths
medicine.disease_cause
Microbiology
Bone and Bones
Pathogenesis
03 medical and health sciences
Bacterial Proteins
medicine
Animals
Humans
Gene
Peroxidase
Comparative genomics
Osteoblasts
Whole Genome Sequencing
Biofilm
Quorum Sensing
Osteomyelitis
General Medicine
Gene Expression Regulation, Bacterial
Staphylococcal Infections
medicine.disease
Interspersed Repetitive Sequences
Quorum sensing
030104 developmental biology
Infectious Diseases
Bacteremia
Biofilms
Protein Kinases
Genome, Bacterial
Subjects
Details
- ISSN :
- 16180607
- Volume :
- 308
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- International journal of medical microbiology : IJMM
- Accession number :
- edsair.doi.dedup.....2e36559d466beffc579140c8ae207838