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The rice VCS1 is identified as a molecular tool to mark and visualize the vegetative cell of pollen
- Source :
- Plant Signal Behav
- Publication Year :
- 2021
- Publisher :
- Informa UK Limited, 2021.
-
Abstract
- Cell-type-specific markers are valuable tools to reveal developmental processes underlying cell differentiation during plant reproduction. Here we report the pollen vegetative cell marker gene VCS1 (Vegetative Cell Specific 1) of rice (Oryza sativa japonica). VCS1 was expressed specifically in late pollen and was predicted to encode a small FAF domain-containing protein of 205 amino acid residues (aa). The expression of reporter fusion proteins showed that VCS1 was exclusively targeted to the vegetative nucleus of pollen. Upon pollen germination, VCS1 lost vegetative nucleus localization, and appeared diffused in the vegetative cytoplasm of pollen grain but not in the pollen tube. T-DNA insertional mutation which disrupted the carboxyl-terminal 21 aa of VCS1 did not affect plant vegetative growth and pollen development, while destruction of VCS1 by CRISPR/Cas9 only moderately affect pollen viability. VCS1 is evolutionally conserved in monocots but appeared absent in dicotyledons. This study reveals a molecular tool for visualizing the vegetative cell of rice and possible other monocots, which has potential values in the genetic engineering of male-sterile lines.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Cytoplasm
Vegetative reproduction
Plant Development
Plant Science
Biology
Genes, Plant
medicine.disease_cause
01 natural sciences
Marker gene
Plant reproduction
03 medical and health sciences
Gene Expression Regulation, Plant
Pollen
Botany
otorhinolaryngologic diseases
medicine
Amino Acid Sequence
Pollination
Plant Proteins
Cell Nucleus
Oryza sativa
Nucleus localization
food and beverages
Cell Differentiation
Oryza
Plants, Genetically Modified
Biological Evolution
030104 developmental biology
Pollen tube
Germ Cells, Plant
Research Paper
010606 plant biology & botany
Subjects
Details
- ISSN :
- 15592324
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Plant Signaling & Behavior
- Accession number :
- edsair.doi.dedup.....2e35123e4a9207a467421fa675c0a03e