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Anti-rotaviral effects of Glycyrrhiza uralensis extract in piglets with rotavirus diarrhea
- Source :
- Virology Journal, Virology Journal, Vol 9, Iss 1, p 310 (2012)
- Publisher :
- Springer Nature
-
Abstract
- Background Since rotavirus is one of the leading pathogens that cause severe gastroenteritis and represents a serious threat to human and animal health, researchers have been searching for cheap, safe, and effective anti-rotaviral drugs. There is a widespread of interest in using natural products as antiviral agents, and among them, licorice derived from Glycyrrhiza spp. has exerted antiviral properties against several viruses. In this study, anti-rotaviral efficacy of Glycyrrhiza uralensis extract (GUE) as an effective and cheaper remedy without side-effects was evaluated in colostrums-deprived piglets after induction of rotavirus diarrhea. Methods Colostrums-deprived piglets were inoculated with porcine rotavirus K85 (G5P[7]) strain. On the onset of diarrhea, piglets were treated with different concentration of GUE. To evaluate the antiviral efficacy of GUE, fecal consistency score, fecal virus shedding and histological changes of the small intestine, mRNA expression levels of inflammation-related cytokines (IL8, IL10, IFN-β, IFN-γ and TNF-α), signaling molecules (p38 and JNK), and transcription factor (NFκB) in the small intestine and spleen were determined. Results Among the dosages (100-400 mg/ml) administrated to animals, 400 mg/ml of GUE cured diarrhea, and markedly improved small intestinal lesion score and fecal virus shedding. mRNA expression levels of inflammation-related cytokines (IL8, IL10, IFN-β, IFN-γ and TNF-α), signaling molecules (p38 and JNK), and transcription factor (NFκB) in the small intestine and spleen were markedly increased in animals with RVA-induced diarrhea, but dose- dependently decreased in GUE treated animals after RVA-induced diarrhea. Conclusions GUE cures rotaviral enteritis by coordinating antiviral and anti-inflammatory effects. Therapy of this herbal medicine can be a viable medication for curing rotaviral enteritis in animals and humans.
- Subjects :
- Rotavirus
Swine
Drug Evaluation, Preclinical
medicine.disease_cause
Plant Roots
law.invention
Feces
law
Intestine, Small
Glycyrrhiza uralensis
Swine Diseases
biology
Anti-Inflammatory Agents, Non-Steroidal
NF-kappa B
Enteritis
Virus Shedding
Diarrhea
Infectious Diseases
Anti-rotaviral drug
Models, Animal
medicine.symptom
MAP Kinase Signaling System
Antiviral Agents
Rotavirus Infections
lcsh:Infectious and parasitic diseases
Cell Line
Virology
medicine
Animals
lcsh:RC109-216
RNA, Messenger
Viral shedding
Glycyrrhiza uralensis extract
Dose-Response Relationship, Drug
Plant Extracts
Tumor Necrosis Factor-alpha
Colostrum
Research
Interleukin-8
biology.organism_classification
medicine.disease
Animals, Newborn
Glycyrrhiza
Phytotherapy
Spleen
Subjects
Details
- Language :
- English
- ISSN :
- 1743422X
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Virology Journal
- Accession number :
- edsair.doi.dedup.....2e3466ee9b36f3f1aa8b408ea4e88094
- Full Text :
- https://doi.org/10.1186/1743-422x-9-310