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Using MALDI-TOF MS to identify mosquitoes collected in Mali and their blood meals
- Source :
- Parasitology, Parasitology, 2018, 145 (9), pp.1170-1182. ⟨10.1017/S0031182018000070⟩, Parasitology, Cambridge University Press (CUP), 2018, 145 (9), pp.1170-1182. ⟨10.1017/S0031182018000070⟩
- Publication Year :
- 2018
- Publisher :
- HAL CCSD, 2018.
-
Abstract
- Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been recently described as an innovative and effective tool for identifying arthropods and mosquito blood meal sources. To test this approach in the context of an entomological survey in the field, mosquitoes were collected from five ecologically distinct areas of Mali. We successfully analysed the blood meals from 651 mosquito abdomens crushed on Whatman filter paper (WFPs) in the field using MALDI-TOF MS. The legs of 826 mosquitoes were then submitted for MALDI-TOF MS analysis in order to identify the different mosquito species. Eight mosquito species were identified, including Anopheles gambiae Giles, Anopheles coluzzii, Anopheles arabiensis, Culex quinquefasciatus, Culex neavei, Culex perexiguus, Aedes aegypti and Aedes fowleri in Mali. The field mosquitoes for which MALDI-TOF MS did not provide successful identification were not previously available in our database. These specimens were subsequently molecularly identified. The WFP blood meal sources found in this study were matched against human blood (n = 619), chicken blood (n = 9), cow blood (n = 9), donkey blood (n = 6), dog blood (n = 5) and sheep blood (n = 3). This study reinforces the fact that MALDI-TOF MS is a promising tool for entomological surveys.
- Subjects :
- 0301 basic medicine
030231 tropical medicine
Biology
Mali
03 medical and health sciences
0302 clinical medicine
Dogs
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Anopheles
parasitic diseases
Animals
Humans
Chromatography
Sheep
fungi
Equidae
3. Good health
Matrix-assisted laser desorption/ionization
Culex
030104 developmental biology
Infectious Diseases
Culicidae
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Animal Science and Zoology
Parasitology
Cattle
Chickens
Blood Chemical Analysis
Subjects
Details
- Language :
- English
- ISSN :
- 00311820 and 14698161
- Database :
- OpenAIRE
- Journal :
- Parasitology, Parasitology, 2018, 145 (9), pp.1170-1182. ⟨10.1017/S0031182018000070⟩, Parasitology, Cambridge University Press (CUP), 2018, 145 (9), pp.1170-1182. ⟨10.1017/S0031182018000070⟩
- Accession number :
- edsair.doi.dedup.....2e28e6ba830b2d3fe2e1acef99c762b7