Additional file 2 of ALG3 contributes to stemness and radioresistance through regulating glycosylation of TGF-β receptor II in breast cancer

Additional file 2: Supplementary Figure 2. (A) ALG3 expression after transduction in MCF-7 and ZR-75-30 cell lines was confirmed by Western blot. (B) Representative images of colony assays, with cells exposed to 0,2,4,6 Gy X ray treatment were as shown. (C) Representative image of local radiation treatment was as shown. (D) Immunohistochemistry image of tumor tissues from ALG3-transduced mice depicts successful upregulation of ALG3. Original magnification, 200x and 400x. scale bar = 50 μm. (E) Immunohistochemistry images showed that tumor tissues from ALG3-transduced mice overexpressing Ki67. Original magnification, 200x and 400x. scale bar = 50 μm. (F) Cleaved caspase-3 activity was performed on tumor tissues from mice. *** P