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Salvianolic acid A inhibits endothelial dysfunction and vascular remodeling in spontaneously hypertensive rats
- Source :
- Life Sciences. 144:86-93
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Aims Despite the numerous pharmacological agents available for hypertension therapy, hypertension-related microvascular remodeling is not resolved, eventually leading to end-organ damage. The aim of the present study was to investigate the protection of salvianolic acid A (SalA) against microvascular remodeling in vitro and in vivo. Main methods Spontaneously hypertensive rats (SHRs) were administered 2.5, 5 or 10 mg/kg SalA via intraperitoneal injection once a day for 4 weeks. The tail-cuff method was applied to monitor blood pressure; the microvascular structure of the retina was detected by hematoxylin–eosin and immunohistochemical staining; the function of mesenteric arteries was measured by DMT wire myography; endothelial cell proliferation was estimated using the Cell Counting Kit-8; endothelial cell migration was evaluated by wound healing and transwell assay; and endothelial cell integrity was detected by transendothelial electrical resistance and permeability assays. Key findings Although no antihypertensive effects of SalA were observed, SalA attenuated the microvascular inward remodeling of the retina and improved microvascular function in the mesenteries in vivo. Further cell experiments confirmed the beneficial effects of SalA on the integrity of the endothelial monolayer in vitro. Significance Salvianolic acid A inhibited endothelial dysfunction and vascular remodeling in spontaneously hypertensive rats. Therefore, salvianolic acid A could be a potential drug therapy to prevent further targeted organ damage induced by vascular remodeling.
- Subjects :
- Male
0301 basic medicine
medicine.medical_specialty
Cell Membrane Permeability
Myocytes, Smooth Muscle
Cell
Vascular Remodeling
Pharmacology
Rats, Inbred WKY
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Caffeic Acids
Cell Movement
In vivo
Rats, Inbred SHR
Internal medicine
Human Umbilical Vein Endothelial Cells
medicine
Animals
Humans
General Pharmacology, Toxicology and Pharmaceutics
Endothelial dysfunction
Mesenteries
Mesenteric arteries
Cell Proliferation
Wound Healing
Electrical impedance myography
business.industry
Microcirculation
Retinal Vessels
General Medicine
medicine.disease
Mesenteric Arteries
Rats
Endothelial stem cell
030104 developmental biology
Endocrinology
medicine.anatomical_structure
Lactates
Endothelium, Vascular
business
Wound healing
Injections, Intraperitoneal
Subjects
Details
- ISSN :
- 00243205
- Volume :
- 144
- Database :
- OpenAIRE
- Journal :
- Life Sciences
- Accession number :
- edsair.doi.dedup.....2e182ed5a9526bd8ad315059358f4f1b