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Therapeutic effects of momordica charantia L. Ethanolic extract on acetic acid-induced ulcerative colitis in rats
- Source :
- Volume: 80, Issue: 2 119-128, European Journal of Biology
- Publication Year :
- 2021
-
Abstract
- © 2021 European Journal of Biology. All rights reserved.Objective: This study aims to investigate the effect of Momordica charantia L. (MoC) ethanolic extract on ulcerative colitis (UC) and was explored in vitro and in vivo. Materials and Methods: The rats were divided into control (C), saline-treated colitis (AA), MoC extract-treated colitis (AA+MoC), and sulfasalazine (SS)-treated colitis (AA+SS) groups. Colitis was induced by acetic acid. MoC extract, SS or saline were given to the related groups for 3 days. Interleukine-1β, malondialdehyde, glutathione levels, myeloperoxidase activity, bax/bcl-2 ratio, caspase-9 and caspase-3 levels were measured in colon and macroscopic and histopathologic examinations were done. Total phenolic/flavonoid content and biological activity of MoC were evaluated by in vitro analysis. Results: Compared to the control group, with acetic acid application interleukin-1β levels, myeloperoxidase activity, malondialdehyde levels, bax/bcl-2 ratio, caspase-9 and caspase-3 levels were significantly upregulated, while glutathione levels were significantly decreased in the AA group. In contrast, MoC and SS treatments reduced interleukin-1β, malondialdehyde levels, myeloperoxidase activity, bax/bcl-2 ratio, and caspase-9 and caspase-3 levels. Glutathione levels increased upon MoC or SS treatment. Increased macroscopic and microscopic scoring with AA improved with MoC or SS treatment, but the MoC or SS treated groups had higher score values than the control. Also, in vitro results showed that MoC exhibited 2,2-diphenyl-1- picrylhydrazyl and 2,2\"-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical scavenging activity as well as significant antilipoxygenase activity. In addition, MoC extract showed a potent anti-inflammatory activity compared to standard indomethacin. Conclusion: Our biochemical, in vitro and histopathologic analysis indicate that MoC is likely to prove beneficial in UC therapy.
- Subjects :
- MICROBIOLOGY
Mikrobiyoloji
Science
Temel Tıp Bilimleri
Histoloji-Embriyoloji
Life Sciences (LIFE)
anti-lipoxygenase activity
Molecular Biology and Genetics
CELL BIOLOGY
Sağlık Bilimleri
radical scavenging activity
Fundamental Medical Sciences
Biochemistry, Genetics and Molecular Biology (miscellaneous)
BİYOKİMYA VE MOLEKÜLER BİYOLOJİ
Yaşam Bilimleri
Health Sciences
Momordica charantia L.,ulcerative colitis,apoptosis,oxidative stress,radical scavenging activity,anti-lipoxygenase activity
oxidative stress
Cytogenetic
Molecular Biology
Moleküler Biyoloji ve Genetik
ulcerative colitis
Fen
Hücre Biyolojisi
Moleküler Biyoloji
Temel Bilimler
Histology and Embryology
apoptosis
Life Sciences
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Biyokimya, Genetik ve Moleküler Biyoloji (çeşitli)
HÜCRE BİYOLOJİSİ
Tıp
MOLECULAR BIOLOGY & GENETICS
BİYOTEKNOLOJİ VE UYGULAMALI MİKROBİYOLOJİ
Momordica charantia L
Yaşam Bilimleri (LIFE)
Medicine
Biyoteknoloji
Natural Sciences
BIOCHEMISTRY & MOLECULAR BIOLOGY
Sitogenetik
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 26022575 and 26186144
- Database :
- OpenAIRE
- Journal :
- Volume: 80, Issue: 2 119-128, European Journal of Biology
- Accession number :
- edsair.doi.dedup.....2e15248e6bffa35665dbf215eb28281e