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A recombinant glucocorticoid-induced leucine zipper protein ameliorates symptoms of dextran sulfate sodium-induced colitis by improving intestinal permeability
- Source :
- Digibug. Repositorio Institucional de la Universidad de Granada, instname
- Publication Year :
- 2021
-
Abstract
- Italian Ministry of Education, Universities and Research, Grant/Award Number: PRIN 2017B9NCSX; Vini di Batasiolo S.p.A.; Junta de Andalucia, Grant/Award Number: CTS 164; Instituto de Salud Carlos III, Grant/Award Number: PI19/1058 and CP19/00191; EMBO<br />Inflammatory bowel diseases (IBDs) are chronic inflammatory disorders characterized by relapsing intestinal inflammation, but many details of pathogenesis remain to be fully unraveled. Glucocorticoid (GC)-induced leucine zipper (GILZ) is a mediator of the anti-inflammatory effects of GCs, the most powerful drugs for IBD treatment, but they cause several unwanted side effects. The fusion protein TAT-GILZ has been successfully used in some pre-clinical models of inflammatory and autoimmune diseases. To test the efficacy of TAT-GILZ for treating dextran sulfate sodium (DSS)-induced colitis and explore its impact on the gut microbiome, colitis was induced by DSS in C57BL/6J mice and treated with TAT-GILZ or dexamethasone. Various hallmarks of colitis were analyzed, including disease activity index, gut permeability, and expression of pro-inflammatory cytokines and tight junction proteins. TAT-GILZ treatment showed a therapeutic effect when administered after the onset of colitis. Its efficacy was associated with improved gut permeability, as evidenced by zonula occludens-1 and CD74 upregulation in inflamed colonic tissue. TAT-GILZ also ameliorated the changes in the gut microbiota induced by the DSS, thus potentially providing an optimal environment for colonization of the mucosa surface by beneficial bacteria. Overall, our results demonstrated for the first time that TAT-GILZ treatment proved effective after disease onset allowing restoration of gut permeability, a key pathogenic feature of colitis. Additionally, TAT-GILZ restored gut dysbiosis, thereby contributing to healing mechanisms. Interestingly, we found unprecedented effects of exogenous GILZ that did not overlap with those of GCs.<br />Ministry of Education, Universities and Research (MIUR) PRIN 2017B9NCSX<br />Vini di Batasiolo S.p.A.<br />Junta de Andalucia CTS 164<br />Instituto de Salud Carlos III European Commission PI19/1058 CP19/00191<br />European Molecular Biology Organization (EMBO)
- Subjects :
- Male
Recombinant Fusion Proteins
Anti-Inflammatory Agents
GILZ
Pharmacology
Gut flora
Biochemistry
Dexamethasone
Permeability
Pathogenesis
Mice
Downregulation and upregulation
microbiota
Genetics
medicine
Animals
Colitis
Intestinal Mucosa
Molecular Biology
Glucocorticoids
Intestinal permeability
glucocorticoids
biology
business.industry
Microbiota
Dextran Sulfate
Histocompatibility Antigens Class II
dysbiosis
medicine.disease
biology.organism_classification
Up-Regulation
Antigens, Differentiation, B-Lymphocyte
Mice, Inbred C57BL
Disease Models, Animal
Treatment Outcome
Trans-Activators
Zonula Occludens-1 Protein
Dysbiosis
Cytokines
colitis, dysbiosis, GILZ, glucocorticoids, microbiota
business
Glucocorticoid
Biotechnology
medicine.drug
Signal Transduction
Transcription Factors
Subjects
Details
- ISSN :
- 15306860
- Volume :
- 35
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- FASEB journal : official publication of the Federation of American Societies for Experimental BiologyREFERENCES
- Accession number :
- edsair.doi.dedup.....2e147e2be455c6888390e9bfca413e2d