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Measuring rat kidney glomerular number and size in vivo with MRI
- Publication Year :
- 2017
- Publisher :
- American Physiological Society, 2017.
-
Abstract
- Nephron number is highly variable in humans and is thought to play an important role in renal health. Chronic kidney disease (CKD) is the result of too few nephrons to maintain homeostasis. Currently, nephron number can only be determined invasively or as a terminal assessment. Due to a lack of tools to measure and track nephron number in the living, the early stages of CKD often go unrecognized, preventing early intervention that might halt the progression of CKD. In this work, we present a technique to directly measure glomerular number ( Nglom) and volume in vivo in the rat kidney ( n = 8) using MRI enhanced with the novel contrast agent cationized ferritin (CFE-MRI). Adult male rats were administered intravenous cationized ferritin (CF) and imaged in vivo with MRI. Glomerular number was measured and each glomerulus was spatially mapped in 3D in the image. Mean apparent glomerular volume (a Vglom) and intrarenal distribution of the individual glomerular volume (IGV), were also measured. These metrics were compared between images of the same kidneys scanned in vivo and ex vivo with CFE-MRI. In vivo Nglom and a Vglom correlated to ex vivo metrics within the same kidneys and were within 10% of Nglom and a Vglom previously validated by stereologic methods. This is the first report of direct in vivo measurements of Nglom and a Vglom, introducing an opportunity to investigate mechanisms of renal disease progression and therapeutic response over time.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Physiology
Kidney Glomerulus
030232 urology & nephrology
Rat kidney
Contrast Media
Nephron
Biology
urologic and male genital diseases
030218 nuclear medicine & medical imaging
Rats, Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Imaging, Three-Dimensional
In vivo
Predictive Value of Tests
Image Interpretation, Computer-Assisted
medicine
Animals
Glomerular volume
urogenital system
Reproducibility of Results
medicine.disease
Magnetic Resonance Imaging
Disease Models, Animal
medicine.anatomical_structure
Ferritins
Innovative Methodology
Kidney Diseases
Homeostasis
Software
Kidney disease
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....2e075cbee8458c317230201a26bd7c10