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Betacellulin regulates the proliferation and differentiation of retinal progenitor cells in vitro
- Source :
- Journal of Cellular and Molecular Medicine
- Publication Year :
- 2017
- Publisher :
- John Wiley and Sons Inc., 2017.
-
Abstract
- Retinal progenitor cells (RPCs) hold great potential for the treatment of retinal degenerative diseases. However, their proliferation capacity and differentiation potential towards specific retinal neurons are limited, which limit their future clinical applications. Thus, it is important to improve the RPCs’ ability to proliferate and differentiate. Currently, epidermal growth factor (EGF) is commonly used to stimulate RPC growth in vitro. In this study, we find that betacellulin (BTC), a member of the EGF family, plays important roles in the proliferation and differentiation of RPCs. Our results showed that BTC can significantly promote the proliferation of RPCs more efficiently than EGF. EGF stimulated RPC proliferation through the EGFR/ErbB2‐Erk pathway, while BTC stimulated RPC proliferation more powerfully through the EGFR/ErbB2/ErbB4‐Akt/Erk pathway. Meanwhile, under differentiated conditions, the BTC‐pre‐treated RPCs were preferentially differentiated into retinal neurons, including photoreceptors, one of the most important types of cells for retinal cell replacement therapy, compared to the EGF‐pre‐treated RPCs. In addition, knockdown of endogenous BTC expression can also obviously promote RPC differentiation into retinal neuronal cells. This data demonstrate that BTC plays important roles in promoting RPC proliferation and differentiation into retinal neurons. This study may provide new insights into the study of RPC proliferation and differentiation and make a step towards the application of RPCs in the treatment of retinal degenerative diseases.
- Subjects :
- 0301 basic medicine
MAPK/ERK pathway
MAP Kinase Signaling System
proliferation
Endogeny
Receptors, Cell Surface
Biology
Retina
03 medical and health sciences
chemistry.chemical_compound
Retinal progenitor
Epidermal growth factor
Animals
Cell Proliferation
Gene knockdown
Betacellulin
Epidermal Growth Factor
Stem Cells
Retinal
Cell Differentiation
Cell Biology
Original Articles
differentiation
In vitro
Cell biology
Culture Media
Mice, Inbred C57BL
030104 developmental biology
chemistry
retinal progenitor cell
Gene Knockdown Techniques
betacellulin
Molecular Medicine
Original Article
sense organs
Proto-Oncogene Proteins c-akt
Retinal Neurons
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 15824934 and 15821838
- Volume :
- 22
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular and Molecular Medicine
- Accession number :
- edsair.doi.dedup.....2dfc076f12693ed538a9137d6634e4ad