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Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injection
- Source :
- Molecular Immunology. 122:62-68
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Among a variety of traditional Chinese medicines, Shuanghuanglian injection (SHLI), has the highest incidence of injection-induced immediate hypersensitivity reactions (IHRs). However, the precise mechanisms of SHLI-induced IHRs remain to be understood. In the present study, we characteriszed IHRs as induced by SHLI by recording changes in physiological and hemodynamic indicators following intravenous injections of SHLI in rats and dogs. The results indicate that SHLI induced the release of histamine, decreased mean arterial blood pressure (MAP), increased SC5b-9 in rats and dogs, increased C4d and Bb in dogs without any changes in IgE. n vitro incubation of SHLI with serum from dogs in the presence of an inhibitor of complement activation (EGTA/Mg2+) resulted in an increase in C4d. These results suggest that SHLI induces anaphylactoid reactions in rats and dogs. Furthermore, SHLI appears to activate the complement system through classical and alternative pathways in dogs in vivo. Additional experiments in mice demonstrated that SHLI induces locus coeruleus infiltration and results in significant increase in vascular permeability within the skin of mice. We established a reliable method for the evaluation of anaphylactoid reactions induced by complex compounds, using multiple physiological indicators, different experimental models in vivo and in vitro.
- Subjects :
- 0301 basic medicine
biology
business.industry
Immunology
Vascular permeability
Pharmacology
Immunoglobulin E
In vitro
Complement system
03 medical and health sciences
EGTA
chemistry.chemical_compound
030104 developmental biology
0302 clinical medicine
chemistry
In vivo
biology.protein
Medicine
Locus coeruleus
business
Molecular Biology
Histamine
030215 immunology
Subjects
Details
- ISSN :
- 01615890
- Volume :
- 122
- Database :
- OpenAIRE
- Journal :
- Molecular Immunology
- Accession number :
- edsair.doi.dedup.....2df292f953e44ba12037099fce2ee278