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Variation of amino acid sequences of serum amyloid a (SAA) and immunohistochemical analysis of amyloid a (AA) in Japanese domestic cats
- Source :
- The Journal of Veterinary Medical Science
- Publication Year :
- 2018
- Publisher :
- 日本獣医学会, 2018.
-
Abstract
- Amyloid A (AA) amyloidosis, a fatal systemic amyloid disease, occurs secondary to chronic inflammatory conditions in humans. Although persistently elevated serum amyloid A (SAA) levels are required for its pathogenesis, not all individuals with chronic inflammation necessarily develop AA amyloidosis. Furthermore, many diseases in cats are associated with the elevated production of SAA, whereas only a small number actually develop AA amyloidosis. We hypothesized that a genetic mutation in the SAA gene may strongly contribute to the pathogenesis of feline AA amyloidosis. In the present study, genomic DNA from four Japanese domestic cats (JDCs) with AA amyloidosis and from five without amyloidosis was analyzed using polymerase chain reaction (PCR) amplification and direct sequencing. We identified the novel variation combination of 45R-51A in the deduced amino acid sequences of four JDCs with amyloidosis and five without. However, there was no relationship between amino acid variations and the distribution of AA amyloid deposits, indicating that differences in SAA sequences do not contribute to the pathogenesis of AA amyloidosis. Immunohistochemical analysis using antisera against the three different parts of the feline SAA protein-i.e., the N-terminal, central, and C-terminal regions-revealed that feline AA contained the C-terminus, unlike human AA. These results indicate that the cleavage and degradation of the C-terminus are not essential for amyloid fibril formation in JDCs.
- Subjects :
- Male
0301 basic medicine
040301 veterinary sciences
Inflammation
Biology
Cat Diseases
0403 veterinary science
Pathogenesis
03 medical and health sciences
Amyloid disease
Japan
AA amyloidosis
Pathology
medicine
Animals
Amino Acid Sequence
Serum amyloid A
Peptide sequence
Serum Amyloid A Protein
Full Paper
General Veterinary
Japanese domestic cat
fibril formation
Amyloidosis
DNA
04 agricultural and veterinary sciences
medicine.disease
Molecular biology
SAA gene
030104 developmental biology
Genes
Cats
Female
medicine.symptom
Subjects
Details
- Language :
- English
- Volume :
- 80
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- The Journal of veterinary medical science
- Accession number :
- edsair.doi.dedup.....2ddf111af2741b992e1b9c3a60d1b119