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Anti-Atherogenic Properties of Allium ursinum Liophylisate: impact on Lipoprotein Homeostasis and Cardiac Biomarkers in Hypercholesterolemic Rabbits
- Source :
- International Journal of Molecular Sciences, Vol 17, Iss 8, p 1284 (2016), International Journal of Molecular Sciences, International Journal of Molecular Sciences; Volume 17; Issue 8; Pages: 1284
- Publication Year :
- 2016
-
Abstract
- The present investigation evaluates the capacity of Allium ursinum (wild garlic) leaf lyophilisate (WGLL; alliin content: 0.261%) to mitigate cardiovascular damage in hypercholesterolemic rabbits. New Zealand rabbits were divided into three groups: (i) cholesterol-free rabbit chow (control); (ii) rabbit chow containing 2% cholesterol (hypercholesterolemic, HC); (iii) rabbit chow containing 2% cholesterol + 2% WGLL (hypercholesterolemic treated, HCT); for eight weeks. At the zero- and eight-week time points, echocardiographic measurements were made, along with the determination of basic serum parameters. Following the treatment period, after ischemia-reperfusion injury, hemodynamic parameters were measured using an isolated working heart model. Western blot analyses of heart tissue followed for evaluating protein expression and histochemical study for the atheroma status determination. WGLL treatment mediated increases in fractional shortening; right ventricular function; peak systolic velocity; tricuspidal annular systolic velocity in live animals; along with improved aortic and coronary flow. Western blot analysis revealed WGLL-associated increases in HO-1 protein and decreases in SOD-1 protein production. WGLL-associated decreases were observed in aortic atherosclerotic plaque coverage, plasma ApoB and the activity of LDH and CK (creatine kinase) in plasma. Plasma LDL was also significantly reduced. The results clearly demonstrate that WGLL has complex cardioprotective effects, suggesting future strategies for its use in prevention and therapy for atherosclerotic disorders.
- Subjects :
- 0301 basic medicine
Male
Apolipoprotein B
Hemodynamics
030204 cardiovascular system & hematology
Allium species
lcsh:Chemistry
chemistry.chemical_compound
0302 clinical medicine
Wild garlic
echocardiography
Elméleti orvostudományok
lcsh:QH301-705.5
Spectroscopy
biology
medicine.diagnostic_test
lipoprotein
cardiovascular homeostasis
General Medicine
Orvostudományok
Computer Science Applications
Rabbits
medicine.medical_specialty
atherosclerosis
Lipoproteins
Hypercholesterolemia
Catalysis
Article
Allium
Inorganic Chemistry
03 medical and health sciences
Western blot
Internal medicine
medicine
Animals
Physical and Theoretical Chemistry
Molecular Biology
Cholesterol
Plant Extracts
Organic Chemistry
biology.organism_classification
medicine.disease
030104 developmental biology
Endocrinology
Atheroma
chemistry
lcsh:Biology (General)
lcsh:QD1-999
biology.protein
Creatine kinase
Biomarkers
Lipoprotein
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences, Vol 17, Iss 8, p 1284 (2016), International Journal of Molecular Sciences, International Journal of Molecular Sciences; Volume 17; Issue 8; Pages: 1284
- Accession number :
- edsair.doi.dedup.....2dddcb5877590a63914312f75295edee