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An immunoaffinity column for the selective purification of 3-methyl-quinoxaline-2-carboxylic acid from swine tissues and its determination by high-performance liquid chromatography with ultraviolet detection and a colloidal gold-based immunochromatographic assay
- Source :
- Food Chemistry. 237:290-296
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- An immunoaffinity column (IAC) for the selective purification of 3-methyl-quinoxaline-2-carboxylic acid (MQCA) from porcine muscle and the liver as well as the methods for its determination by high-performance liquid chromatography with ultraviolet detection (HPLC-UV) and a colloidal gold-based immunochromatographic assay (GICA) were developed. This is the first study that describes a clean-up method that employs an IAC for the determination of MQCA. Using the HPLC-UV method, the limits of detection and limits of quantification for MQCA in different samples were 1.0–3.0 μg kg −1 and 4.0–10.0 μg kg −1 , respectively. The average recoveries of MQCA that was spiked into samples at concentrations of 4.0–100 μg kg −1 were 80.1–87.7%, with relative standard deviations of less than 8.5%. Using the GICA method, the limits of detection for MQCA in different samples were 10 μg kg −1 and 50 μg kg −1 . No false negatives or false positives were observed.
- Subjects :
- Detection limit
Chromatography
Swine
010405 organic chemistry
Chemistry
Muscles
Quinoxaline-2-carboxylic acid
010401 analytical chemistry
Porcine muscle
Gold Colloid
General Medicine
01 natural sciences
High-performance liquid chromatography
Chromatography, Affinity
0104 chemical sciences
Analytical Chemistry
Colloidal gold
Quinoxalines
Animals
Chromatography, High Pressure Liquid
Food Science
Subjects
Details
- ISSN :
- 03088146
- Volume :
- 237
- Database :
- OpenAIRE
- Journal :
- Food Chemistry
- Accession number :
- edsair.doi.dedup.....2ddbed7239f1807b003c71fbd55c2f4c