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Inhibitory effects of the herbal medicine Sho-saiko-to (TJ-9) on cell proliferation and procollagen gene expressions in cultured rat hepatic stellate cells
- Source :
- Journal of Hepatology. 29:642-649
- Publication Year :
- 1998
- Publisher :
- Elsevier BV, 1998.
-
Abstract
- It is of extreme importance to prevent liver fibrosis and subsequent progression to liver cirrhosis. The aim of our study was to elucidate in vitro whether Sho-saiko-to exerted inhibitory effects on hepatic stellate cells.Hepatic stellate cells were isolated from male Wistar rats. Water-soluble ingredients of Sho-saiko-to were obtained at concentrations of 10, 100, 250, 500 and 1000 microg/ml. Morphological transformation was observed under a phase-contrast microscope. Flow cytometric analysis was performed on day 4 after culture to evaluate the potential to proliferate of the stellate cells by analyzing cell cycles. Northern blot analysis was carried out on day 3 after culture to determine the expressions of type I and type III procollagen mRNAs.(i) Sho-saiko-to 500 and 1000 microg/ml inhibited morphological transformation of the stellate cells to myofibroblast-like cells. (ii) Sho-saiko-to 500 and 1000 microg/ml significantly (p0.0001) accumulated the cells in the G0/G1 phase (118.8+/-0.7%, 119.2+/-0.5%, respectively as compared with control) and significantly (p0.0001) decreased cell numbers subsequently in G2/M phase (47.5+/-8.1%, 48.9+/-2.0%, respectively). (iii) Sho-saiko-to 500 and 1000 microg/ml also significantly (p0.05 or p0.0001) suppressed procollagen mRNA expression of type I to 51.5+/-6.4%, 34.9+/-3.7%, respectively, and type III to 51.3+/-12.3%, 46.7+/-11.4%, respectively.We have clarified the inhibitory effects of Sho-saiko-to on hepatic stellate cells in vitro. Sho-saiko-to could be a potent inhibitor in the pathogenesis of liver fibrosis.
- Subjects :
- Liver Cirrhosis
Male
Pathology
medicine.medical_specialty
Cirrhosis
Liver cytology
Fibrosis
Adipocytes
medicine
Animals
RNA, Messenger
Rats, Wistar
Cells, Cultured
Sho-saiko-to
Hepatology
Cell growth
business.industry
Cell Cycle
Flow Cytometry
medicine.disease
Molecular biology
Rats
Procollagen peptidase
Liver
Cell culture
Hepatic stellate cell
business
Cell Division
Procollagen
Drugs, Chinese Herbal
Subjects
Details
- ISSN :
- 01688278
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- Journal of Hepatology
- Accession number :
- edsair.doi.dedup.....2da499593ca7a4d2e2a5cf4e3fe90477