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Moxifloxacin resistance and genotyping of Mycobacterium avium and Mycobacterium intracellulare isolates in Japan
- Source :
- Journal of Infection and Chemotherapy. 25:995-1000
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Background Although fluoroquinolones are considered as alternative therapies of pulmonary Mycobacterium avium complex (MAC) disease, the association between fluoroquinolone resistance and MAC genotypes in clinical isolates from individuals not previously treated for MAC infection is not fully clear. Methods Totals of 154 M. avium isolates and 35 Mycobacterium intracellulare isolates were obtained from treatment-naive patients with pulmonary MAC disease at the diagnosis of MAC infection at 8 hospitals in Japan. Their susceptibilities of moxifloxacin were determined by broth microdilution methods. Moxifloxacin-resistant isolates were examined for mutations of gyrA and gyrB. Variable numbers of tandem repeats (VNTR) assay was performed using 15 M. avium VNTR loci and 16 M. intracellulare VNTR loci. Results Moxifloxacin susceptibility was categorized as resistant and intermediate for 6.5% and 16.9%, respectively, of M. avium isolates and 8.6% and 17.1% of M. intracellulare isolates. Although the isolates of both species had amino acid substitutions of Thr 96 and Thr 522 at the sites corresponding to Ser 95 in the M. tuberculosis GyrA and Gly 520 in the M. tuberculosis GyrB, respectively, these substitutions were observed irrespective of susceptibility and did not confer resistance. The VNTR assays showed revealed three clusters among M. avium isolates and two clusters among M. intracellulare isolates. No significant differences in moxifloxacin resistance were observed among these clusters. Conclusions Although resistance or intermediate resistance to moxifloxacin was observed in approximately one-fourth of M. avium and M. intracellulare isolates, this resistance was not associated with mutations in gyrA and gyrB or with VNTR genotypes.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Genotype
Moxifloxacin
030106 microbiology
Microbial Sensitivity Tests
Minisatellite Repeats
Microbiology
03 medical and health sciences
0302 clinical medicine
Japan
Tandem repeat
Drug Resistance, Bacterial
medicine
Humans
Pharmacology (medical)
Mycobacterium avium complex
030212 general & internal medicine
Genotyping
Mycobacterium avium-intracellulare Infection
biology
Broth microdilution
biochemical phenomena, metabolism, and nutrition
Mycobacterium avium Complex
bacterial infections and mycoses
biology.organism_classification
Fluoroquinolone resistance
Anti-Bacterial Agents
Infectious Diseases
DNA Gyrase
Mutation
bacteria
Mycobacterium avium
Mycobacterium
medicine.drug
Subjects
Details
- ISSN :
- 1341321X
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Journal of Infection and Chemotherapy
- Accession number :
- edsair.doi.dedup.....2da05dbf5bd14e4cb1e0130ac36a1c3f