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Cardiomyocyte apoptosis in the right auricle of patients with ostium secundum atrial septal defect diseases
- Source :
- Life sciences. 80(12)
- Publication Year :
- 2006
-
Abstract
- Ostium secundum atrial septal defect (osASD) is one of the most commonly occurring cardiac malformations. Although some embryological pathways have been elucidated, the molecular etiologies of ASD are not fully understood. Previous microarray analysis in our laboratory identified differentially expressed genes between osASD and normal right auricular myocardium. Of the 1056 differentially expressed genes, 14 genes were related to apoptosis: eight pro-apoptotic genes were up-regulated and six anti-apoptotic genes were down-regulated in ASD patients. In the current study, we utilized semi-quantitative RT-PCR, electron microscopy, TUNEL and flow cytometry to further understand the role of apoptosis in the atrium of osASD patients. RT-PCR results confirmed differential expression data from previous microarray studies. Additionally, while apoptosis was detected in the right auricular myocardium of all osASD patients, it was absent in controls. These data suggested apoptosis may play an important role in the pathogenesis of osASD or possibly occurs as a consequence of volume overload and hemodynamic changes in right atrium of osASD patients.
- Subjects :
- Male
medicine.medical_specialty
Pathology
Microarray
Volume overload
Hemodynamics
Down-Regulation
Gene Expression
Apoptosis
Biology
General Biochemistry, Genetics and Molecular Biology
Heart Septal Defects, Atrial
Pathogenesis
Internal medicine
medicine
In Situ Nick-End Labeling
Humans
Myocytes, Cardiac
Heart Atria
General Pharmacology, Toxicology and Pharmaceutics
Atrium (heart)
Oligonucleotide Array Sequence Analysis
TUNEL assay
Microarray analysis techniques
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
General Medicine
Flow Cytometry
Up-Regulation
Microscopy, Electron
medicine.anatomical_structure
cardiovascular system
Cardiology
Subjects
Details
- ISSN :
- 00243205
- Volume :
- 80
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Life sciences
- Accession number :
- edsair.doi.dedup.....2d9bf6d20f5948aec08a846b83d3a884