Back to Search
Start Over
Impact of weight loss and partial weight regain on immune cell and inflammatory markers in adipose tissue in male mice
- Source :
- J Appl Physiol (1985)
- Publication Year :
- 2020
-
Abstract
- Weight fluctuations are common among individuals with obesity and are associated with increased morbidity. We examined adipose tissue immune and inflammatory markers in mice following weight loss and partial weight regain. Male C57BL/6 mice were randomized into four groups (n = 8–10/group): low-fat diet for 32 wk (LFD), high-fat diet for 32 wk (HFD), LFD for 28 wk and then changed to a HFD for 4 wk (LFD→H), and HFD for 21 wk and then changed to LFD for 7 wk and then changed to HFD for 4 wk (HFD→L→H). LFD→H and HFD→L→H mice did not differ in body weight, fat mass, or fat percentage; however, these parameters were greater than in LFD (P < 0.05) but lower than in HFD (P < 0.05). HFD→L→H mice had smaller adipocytes than HFD and LFD→H (P < 0.05) but not LFD mice. Expressions of CD11c and CD8a genes were elevated in epididymal fat of HFD→L→H compared with LFD→H and LFD (P < 0.05)mice. However, CD11c was lower in HFD→L→H than in HFD mice (P < 0.05), but there was no difference in CD8a between these groups. TNFα and IFNγ expressions were increased in HFD→L→H compared with LFD and LFD→H mice (P < 0.05), although HFD→L→H had lower expression of these cytokines than HFD (P < 0.05). IL-1β was greater in HFD→L→H compared with LFD (P < 0.05) but was not different from LFD→H or HFD mice. Monocyte chemoattractant protein-1 was lower (P < 0.05) in HFD→L→H than in LFD→H. These data reinforce the importance of maintaining a body weight in the range that is recommended for optimal health to reduce immune and inflammatory perturbations associated with obesity. NEW & NOTEWORTHY We examined the immune and inflammatory status of adipose tissue in mice after they underwent weight loss followed by partial weight regain. We show an increase in selected immune cells and inflammatory mediators, in high-fat diet-fed mice that had prior exposure to a high-fat diet. Although weight fluctuations appear to exacerbate immune cell abundance and inflammation in adipose tissue, severity is less than in mice that were exposed to sustained high-fat diet feedings.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
Physiology
Adipose tissue
030209 endocrinology & metabolism
Inflammation
Diet, High-Fat
Weight Gain
03 medical and health sciences
Mice
0302 clinical medicine
Immune system
Weight loss
Physiology (medical)
Internal medicine
Weight Loss
Medicine
Animals
business.industry
Monocyte
digestive, oral, and skin physiology
Body Weight
nutritional and metabolic diseases
food and beverages
Weight Fluctuation
medicine.disease
Obesity
Mice, Inbred C57BL
030104 developmental biology
medicine.anatomical_structure
Endocrinology
Adipose Tissue
Tumor necrosis factor alpha
medicine.symptom
business
Research Article
Subjects
Details
- ISSN :
- 15221601
- Volume :
- 129
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Journal of applied physiology (Bethesda, Md. : 1985)
- Accession number :
- edsair.doi.dedup.....2d94e2403a26c564f419ef0733412f2c