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Type 2 diabetes increases oocyte mtDNA mutations which are eliminated in the offspring by bottleneck effect
- Source :
- Reproductive Biology and Endocrinology : RB&E, Reproductive Biology and Endocrinology, Vol 16, Iss 1, Pp 1-8 (2018)
- Publication Year :
- 2018
- Publisher :
- BioMed Central, 2018.
-
Abstract
- Background Diabetes induces many complications including reduced fertility and low oocyte quality, but whether it causes increased mtDNA mutations is unknown. Methods We generated a T2D mouse model by using high-fat-diet (HFD) and Streptozotocin (STZ) injection. We examined mtDNA mutations in oocytes of diabetic mice by high-throughput sequencing techniques. Results T2D mice showed glucose intolerance, insulin resistance, low fecundity compared to the control group. T2D oocytes showed increased mtDNA mutation sites and mutation numbers compared to the control counterparts. mtDNA mutation examination in F1 mice showed that the mitochondrial bottleneck could eliminate mtDNA mutations. Conclusions T2D mice have increased mtDNA mutation sites and mtDNA mutation numbers in oocytes compared to the counterparts, while these adverse effects can be eliminated by the bottleneck effect in their offspring. This is the first study using a small number of oocytes to examine mtDNA mutations in diabetic mothers and offspring. Electronic supplementary material The online version of this article (10.1186/s12958-018-0423-1) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
Male
Mitochondrial DNA
Oocyte
lcsh:QH471-489
endocrine system diseases
Pregnancy Rate
Offspring
Inheritance Patterns
Type 2 diabetes
Biology
medicine.disease_cause
Diet, High-Fat
lcsh:Gynecology and obstetrics
DNA, Mitochondrial
Bottleneck
Diabetes Mellitus, Experimental
Andrology
03 medical and health sciences
Endocrinology
Insulin resistance
Pregnancy
Diabetes mellitus
medicine
lcsh:Reproduction
Animals
lcsh:RG1-991
Mutation
Research
Diabetes
nutritional and metabolic diseases
Obstetrics and Gynecology
mtDNA mutation
High-Throughput Nucleotide Sequencing
Streptozotocin
medicine.disease
Mice, Inbred C57BL
030104 developmental biology
medicine.anatomical_structure
Fertility
Reproductive Medicine
Diabetes Mellitus, Type 2
Oocytes
Female
Developmental Biology
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 14777827
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Reproductive Biology and Endocrinology : RB&E
- Accession number :
- edsair.doi.dedup.....2d5a777be90e747afb9e643a73393987