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Single cell versus large population analysis: cell variability in elemental intracellular concentration and distribution
- Source :
- Analytical & bioanalytical chemistry, (2017): 1–12. doi:10.1007/s00216-017-0725-8, info:cnr-pdr/source/autori:Malucelli, Emil; Procopio, Alessandra; Fratini, Michela; Fratini, Michela; Gianoncelli, Alessandra; Notargiacomo, Andrea; Merolle, Lucia; Sargenti, Azzurra; Castiglioni, Sara; Cappadone, Concettina; Farruggia, Giovanna; Farruggia, Giovanna; Lombardo, Marco; Lagomarsino, Stefano; Maier, Jeanette A.; Iotti, Stefano; Iotti, Stefano/titolo:Single cell versus large population analysis: cell variability in elemental intracellular concentration and distribution/doi:10.1007%2Fs00216-017-0725-8/rivista:Analytical & bioanalytical chemistry (Print)/anno:2017/pagina_da:1/pagina_a:12/intervallo_pagine:1–12/volume
- Publication Year :
- 2017
- Publisher :
- Springer, Heidelberg , Germania, 2017.
-
Abstract
- The quantification of elemental concentration in cells is usually performed by analytical assays on large populations missing peculiar but important rare cells. The present article aims at comparing the elemental quantification in single cells and cell population in three different cell types using a new approach for single cells elemental analysis performed at sub-micrometer scale combining X-ray fluorescence microscopy and atomic force microscopy. The attention is focused on the light element Mg, exploiting the opportunity to compare the single cell quantification to the cell population analysis carried out by a highly Mg-selective fluorescent chemosensor. The results show that the single cell analysis reveals the same Mg differences found in large population of the different cell strains studied. However, in one of the cell strains, single cell analysis reveals two cells with an exceptionally high intracellular Mg content compared with the other cells of the same strain. The single cell analysis allows mapping Mg and other light elements in whole cells at sub-micrometer scale. A detailed intensity correlation analysis on the two cells with the highest Mg content reveals that Mg subcellular localization correlates with oxygen in a different fashion with respect the other sister cells of the same strain. Graphical abstract Single cells or large population analysis this is the question!
- Subjects :
- 0301 basic medicine
Cell type
Cell
Population
Cell Count
01 natural sciences
Biochemistry
Analytical Chemistry
Light elements quantification
Single cells analysi
03 medical and health sciences
Single-cell analysis
Single cells analysis
Cell Line, Tumor
Fluorescence microscope
medicine
Human Umbilical Vein Endothelial Cells
Humans
Magnesium
education
Synchrotron-based X-ray fluorescence microscopy
Fluorescent Dyes
education.field_of_study
Chemistry
X-Rays
010401 analytical chemistry
Optical Imaging
Fluorescence
0104 chemical sciences
030104 developmental biology
medicine.anatomical_structure
Microscopy, Fluorescence
Fluorescent chemosensors
Elemental analysis
Biophysics
Single-Cell Analysis
Intracellular
Synchrotrons
Fluorescent chemosensor
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Analytical & bioanalytical chemistry, (2017): 1–12. doi:10.1007/s00216-017-0725-8, info:cnr-pdr/source/autori:Malucelli, Emil; Procopio, Alessandra; Fratini, Michela; Fratini, Michela; Gianoncelli, Alessandra; Notargiacomo, Andrea; Merolle, Lucia; Sargenti, Azzurra; Castiglioni, Sara; Cappadone, Concettina; Farruggia, Giovanna; Farruggia, Giovanna; Lombardo, Marco; Lagomarsino, Stefano; Maier, Jeanette A.; Iotti, Stefano; Iotti, Stefano/titolo:Single cell versus large population analysis: cell variability in elemental intracellular concentration and distribution/doi:10.1007%2Fs00216-017-0725-8/rivista:Analytical & bioanalytical chemistry (Print)/anno:2017/pagina_da:1/pagina_a:12/intervallo_pagine:1–12/volume
- Accession number :
- edsair.doi.dedup.....2d4c783dd7eb8c01572273da65fcc0d7