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Linear Assembly of a Human Y Centromere

Authors :
Karen H. Miga
Mark Akeson
David Haussler
Huntington F. Willard
Kira V. Bulazel
David Stoddart
Miten Jain
Daniel J. Turner
Benedict Paten
Hugh E. Olsen
Publication Year :
2017
Publisher :
Cold Spring Harbor Laboratory, 2017.

Abstract

The human genome reference sequence remains incomplete due to the challenge of assembling long tracts of near-identical tandem repeats-in centromeric regions. To address this, we have implemented a nanopore sequencing strategy to generate high quality reads that span hundreds of kilobases of highly repetitive DNAs. Here, we use this advance to produce a sequence assembly and characterization of the centromeric region of a human Y chromosome.

Details

Language :
English
Database :
OpenAIRE
Accession number :
edsair.doi.dedup.....2d46845b4473883504e3c365c37fa39b
Full Text :
https://doi.org/10.1101/170373