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Titer on Chip: New Analytical Tool for Influenza Vaccine Potency Determination
- Source :
- PLoS ONE, PLoS ONE, Vol 9, Iss 10, p e109616 (2014)
- Publication Year :
- 2014
- Publisher :
- Public Library of Science, 2014.
-
Abstract
- Titer on Chip (Flu-ToC) is a new technique for quantification of influenza hemagglutinin (HA) concentration. In order to evaluate the potential of this new technique, a comparison of Flu-ToC to more conventional methods was conducted using recombinant HA produced in a baculovirus expression system as a test case. Samples from current vaccine strains were collected from four different steps in the manufacturing process. A total of 19 samples were analysed by Flu-ToC (blinded), single radial immunodiffusion (SRID), an enzyme-linked immunosorbent assay (ELISA), and the purity adjusted bicinchoninic acid assay (paBCA). The results indicated reasonable linear correlation between Flu-ToC and SRID, ELISA, and paBCA, with regression slopes of log-log plots being 0.91, 1.03, and 0.91, respectively. The average ratio for HA content measured by Flu-ToC relative to SRID, ELISA, and paBCA was 83%, 147%, and 81%, respectively; indicating nearly equivalent potency determination for Flu-ToC relative to SRID and paBCA. These results, combined with demonstrated multiplexed analysis of all components within a quadrivalent formulation and robust response to HA strains over a wide time period, support the conclusion that Flu-ToC can be used as a reliable and time-saving alternative potency assay for influenza vaccines.
- Subjects :
- Influenza Viruses
Viral Diseases
Influenza vaccine
Immunology
lcsh:Medicine
Hemagglutinin Glycoproteins, Influenza Virus
Biology
Microbiology
Species Specificity
medicine
Medicine and Health Sciences
Potency
Bicinchoninic acid assay
Animals
lcsh:Science
Microbial Pathogens
Vaccine Potency
Radial immunodiffusion
Immunoassay
Vaccines
Multidisciplinary
Chromatography
medicine.diagnostic_test
lcsh:R
Organisms
Biology and Life Sciences
Hemagglutinin
Virology
Vaccination and Immunization
Influenza
Recombinant Proteins
3. Good health
Titer
Infectious Diseases
Medical Microbiology
Influenza Vaccines
Viral Pathogens
Viruses
lcsh:Q
Baculoviridae
Research Article
Orthomyxoviruses
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 9
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....2d44ccb988a4a0914c66c3aa8579294a