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Cloning of Mongolian gerbil cDNAs encoding inflammatory proteins, and their expression in glandular stomach during H. pylori infection
- Source :
- Cancer science. 95(10)
- Publication Year :
- 2004
-
Abstract
- Mongolian gerbils are considered to be a good animal model for understanding the development of Helicobacter pylori-associated diseases. However, limitations regarding the genetic information available for this animal species hamper the elucidation of underlying mechanisms. Thus, we have focused on identifying the nucleotide sequences of cDNAs encoding Mongolian gerbil inflammatory proteins, such as interleukin-1 (IL-1beta), tumor necrosis factor alpha (TNF-alpha), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Furthermore, we examined the mRNA expression of these genes in the glandular stomach by RT-PCR at 1-8 weeks after H. pylori infection. The deduced amino acid homologies to mouse, rat and human proteins were 86.2%, 83.6% and 67.8% for IL-1beta, 87.2%, 85.1% and 78.4% for TNF-alpha , 91.9%, 90.2% and 84.8% for COX-2 and 90.8%, 89.1% and 80.1% for iNOS, respectively. The average stomach weight of Mongolian gerbils inoculated with H. pylori was increased in a time-dependent manner at 1, 2, 4 and 8 weeks after inoculation. In the pyloric region, mRNA expression levels of IL-1beta, TNF-alpha and iNOS were increased in H. pylori-infected animals at the 2 weeks time point, while in the fundic region, expression levels of IL-1beta, TNF-alpha and iNOS were elevated at 4 and 8 weeks. The COX-2 expression level in the fundic region was clearly elevated in infected animals compared with control animals at 4 and 8 weeks, but in the pyloric region, expression levels were similar in both infected and control animals. Thus, our results indicate that oxidative stress occurs from an early stage of H. pylori infection in the glandular stomach of Mongolian gerbils.
- Subjects :
- Cancer Research
Pathology
medicine.medical_specialty
DNA, Complementary
Interleukin-1beta
Nitric Oxide Synthase Type II
Inflammation
Biology
Gerbil
medicine.disease_cause
Helicobacter Infections
parasitic diseases
medicine
Animals
Helicobacter
Pyloric region
Cloning, Molecular
Helicobacter pylori
Tumor Necrosis Factor-alpha
Stomach
General Medicine
Organ Size
Macrophage Inflammatory Proteins
biology.organism_classification
Molecular biology
Peptide Fragments
Nitric oxide synthase
Isoenzymes
medicine.anatomical_structure
Oncology
Cyclooxygenase 2
Gastric Mucosa
Prostaglandin-Endoperoxide Synthases
Gastritis
biology.protein
Tumor necrosis factor alpha
medicine.symptom
Nitric Oxide Synthase
Gerbillinae
Oxidative stress
Interleukin-1
Subjects
Details
- ISSN :
- 13479032
- Volume :
- 95
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Cancer science
- Accession number :
- edsair.doi.dedup.....2d2d4aef5208933b86f23308419a175a