Back to Search
Start Over
Modification of Wnt signaling pathway on paraquat-induced inhibition of neural progenitor cell proliferation
- Source :
- Food and Chemical Toxicology. 121:311-325
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Paraquat (PQ) is an agricultural chemical used worldwide. As a potential neurotoxicant, PQ adversely affects neurogenesis and inhibits proliferation of neural progenitor cells (NPCs). However, the molecular mechanistic insights of PQ exposure on NPCs remains to be determined. Herein, we determine the extent to which Wnt/β-catenin signaling involved in the inhibition effect of PQ on mouse NPCs from subventricular zone (SVZ). NPCs were treated with different concentrations of PQ (40, 80, and 120 μM). PQ exposure provoked oxidative stress and apoptosis and PQ inhibited cell viability and proliferation in a concentration-dependent manner. Significantly, PQ exposure altered the expression/protein levels of the Wnt pathway genes in NPCs. In addition, PQ reduced cellular β-catenin, p-GSK-3β, and cyclin-D1 and increased the radio of Bax/Bcl2. Further, Wnt pathway activation by treatment with LiCl and Wnt1 attenuated PQ-induced inhibition of mNPCs proliferation. Antioxidant (NAC) treatment alleviated the inhibition of PQ-induced Wnt signaling pathway. Overall, our results suggest significant inhibitory effects of PQ on NPCs proliferation via the Wnt/β-catenin signaling pathway. Interestingly, our results implied that activation of Wnt/β-catenin signaling pathway attenuated PQ-induced autophagic cell death. Our results therefore bring our understanding of the molecular mechanisms of PQ-induced neurotoxicity.
- Subjects :
- Paraquat
0301 basic medicine
Programmed cell death
Cell Survival
Subventricular zone
Apoptosis
Toxicology
Article
Mice
03 medical and health sciences
0302 clinical medicine
Neural Stem Cells
Lateral Ventricles
Autophagy
medicine
Animals
Progenitor cell
Wnt Signaling Pathway
beta Catenin
Cell Proliferation
Herbicides
Chemistry
Neurogenesis
Wnt signaling pathway
General Medicine
Neural stem cell
Cell biology
Mice, Inbred C57BL
Oxidative Stress
030104 developmental biology
medicine.anatomical_structure
Gene Expression Regulation
Signal transduction
030217 neurology & neurosurgery
Food Science
Subjects
Details
- ISSN :
- 02786915
- Volume :
- 121
- Database :
- OpenAIRE
- Journal :
- Food and Chemical Toxicology
- Accession number :
- edsair.doi.dedup.....2d295b4490f075fd93dee73cbec54169