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Dynamic nuclear polarization magnetic resonance imaging and the oxygen-sensitive paramagnetic agent OX63 provide a noninvasive quantitative evaluation of kidney hypoxia in diabetic mice
- Source :
- Kidney international. 96(3)
- Publication Year :
- 2018
-
Abstract
- Renal hypoxia may play an important role in the progression of diabetic nephropathy. However, tools that noninvasively and quantitatively measure oxygen tension in the kidney are lacking. Here, we evaluated the feasibility of a noninvasive and quantitative imaging technique using dynamic nuclear polarization magnetic resonance imaging (DNP-MRI) in combination with the oxygen-sensitive paramagnetic agent OX63 for measuring oxygen tension in the kidney. Our results demonstrate that the DNP-MRI technique can yield quantitative maps of oxygen tension in the mouse renal cortex. Using this procedure, we also showed that oxygen tension was less elevated in the renal cortex of both streptozotocin-induced type 1 diabetic mice and db/db mice, a model of type 2 diabetes, than in the renal cortex of age-matched control mice of each respective model. Oxygen tension in streptozotocin-exposed mice was significantly improved by insulin treatment. Thus, the noninvasive and quantitative DNP-MRI technique appears to be useful for studying the pathophysiological role of hypoxia.
- Subjects :
- 0301 basic medicine
Pathology
medicine.medical_specialty
Kidney Cortex
Renal cortex
medicine.medical_treatment
030232 urology & nephrology
chemistry.chemical_element
Type 2 diabetes
Oxygen
Streptozocin
Diabetes Mellitus, Experimental
Diabetic nephropathy
03 medical and health sciences
Mice
0302 clinical medicine
medicine
Animals
Humans
Diabetic Nephropathies
Kidney
medicine.diagnostic_test
Chemistry
Insulin
Magnetic resonance imaging
Trityl Compounds
medicine.disease
Magnetic Resonance Imaging
Cell Hypoxia
Oxygen tension
Disease Models, Animal
030104 developmental biology
medicine.anatomical_structure
Diabetes Mellitus, Type 1
Indenes
Nephrology
Subjects
Details
- ISSN :
- 15231755
- Volume :
- 96
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Kidney international
- Accession number :
- edsair.doi.dedup.....2d1915af2afa78f3cd9fba85f73de67e