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Community‐acquired bacteremic pneumococcal pneumonia in children: diagnosis and serotyping by real‐time polymerase chain reaction using blood samples
- Source :
- Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 51(9)
- Publication Year :
- 2010
-
Abstract
- Background. The aim of this study was to use real-time polymerase chain reaction (RT-PCR) on blood samples to diagnose and serotype pneumococcal infection in a large cohort of Italian children hospitalized for community-acquired pneumonia. Methods. We conducted an observational study from April 2007 through June 2009 of children aged 0-16 years with a diagnosis of community-acquired pneumonia admitted to 83 pediatric hospitals in Italy. Results. Seven hundred fifty-three children were studied. RT-PCR found pneumococcal infection in 80 (10.6%) of 753 patients. In 292 patients, culture and RT-PCR were simultaneously performed. Streptococcus pneumoniae was identified in 47 of 292 patients; 45 (15.4%) tested positive by RT-PCR and 11 (3.8%) tested positive by culture. RT-PCR was significantly more sensitive than culture in revealing bacteremic pneumonia (odds ratio, 30.6; 95% confidence interval, 5.8-97.5; P < .001). Complicated pneumonia was found in 162 (21.5%) of 753 children; 152 (93.8%) of these 162 had parapneumonic effusion, and 51 (33.6%) had empyema. Children with complicated pneumonia were significantly older. Pneumococcal bacteremia was found by RT-PCR to occur significantly more frequently in children with complications (38 [23.5%] of 162) than in children with uncomplicated pneumonia (44 [7.4%] of 591; odds ratio, 3.8; 95% confidence interval, 2.30-6.30; P
- Subjects :
- Microbiology (medical)
Male
medicine.medical_specialty
Adolescent
Bacteremia
medicine.disease_cause
Polymerase Chain Reaction
Parapneumonic effusion
Community-acquired pneumonia
Bacterial Typing Technique
Internal medicine
Streptococcus pneumoniae
medicine
Humans
Community-Acquired Infection
Serotyping
Preschool
Child
business.industry
Bacterial pneumonia
Infant, Newborn
Infant
Odds ratio
Pneumonia
Pneumonia, Pneumococcal
medicine.disease
Newborn
Bacterial Typing Techniques
Community-Acquired Infections
Infectious Diseases
Blood
Italy
Child, Preschool
Pneumococcal pneumonia
Immunology
Pneumococcal
Female
business
Human
Subjects
Details
- ISSN :
- 15376591
- Volume :
- 51
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
- Accession number :
- edsair.doi.dedup.....2d169097de5946bfcec3fc24267ee5fe