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Biosynthesis of gold nanoparticles using Vetex negundo and evaluation of pro-apoptotic effect on human gastric cancer cell lines
- Source :
- Journal of Photochemistry and Photobiology B: Biology. 203:111749
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Gastric cancer (GC) is mainly widespread gastrointestinal malignancy,which reports for 8% of overallcases in carcinogenesis and 10% of yearly fatality, is 4thprimary cause of cancer associated death global. The plan of the present research was to develop ethanolic extract of Vitex negundo-loaded gold nanoparticles (VN-AuNPs) and to appraise the various characteristic methods likes UV-vis spectroscopy, SAED, FTIR, XRD and HR-TEM. Additionally, the anticancer effect of VN-AuNPs on AGS cells were analysed by cell viability, apoptotic morphological changes by TUNEL, AO/EtBr and Hoechst staining, alterations of mitochondrial membrane potential (MMP) and production reactive oxygen species (ROS). Moreover, the status of apoptosis gene such as caspase-3, Bcl-2, Bcl-XL, Bax and caspase-9 expressions was analysed by using western and RT-PCR techniques. Synthesized AuNPs established by UV absorption peak of the highest at 538 and crystal nature of AuNPs was additionallyverifiedwith SAED and XRD. TEM images were illustrates size and morphological division of NPs. FTIR examinationscompletedalkene, carbodiimide and aliphatic primary amines of biomolecules werepresent in synthesized VN-AuNPs. Additionally, AuNPs were stimulatedapoptosis throughthe cytotoxicity effect,changes of MMP, generation of ROS, nuclear and apoptotic morphological alterationsvia TUNEL, AO/EtBr and Hoechst assay. Furthermore, molecular mechanisms also provoked apoptosis through modulating pro (caspase-3, Bax, Bid, caspase-9) and anti-apoptotic (Bcl-2 and Bcl-XL) mediators by western blotting and gene expression in AGS cells. This production of AuNPs from VN was eco-friendly, large-scaled up and easy.
- Subjects :
- 030303 biophysics
Biophysics
Metal Nanoparticles
Apoptosis
02 engineering and technology
medicine.disease_cause
Vitex
03 medical and health sciences
Stomach Neoplasms
Cell Line, Tumor
Gene expression
medicine
Humans
Radiology, Nuclear Medicine and imaging
Viability assay
Cytotoxicity
Membrane Potential, Mitochondrial
chemistry.chemical_classification
0303 health sciences
Reactive oxygen species
Radiation
TUNEL assay
Radiological and Ultrasound Technology
Caspase 3
Plant Extracts
Green Chemistry Technology
021001 nanoscience & nanotechnology
Molecular biology
Caspase 9
Blot
Proto-Oncogene Proteins c-bcl-2
chemistry
Gold
Reactive Oxygen Species
0210 nano-technology
Carcinogenesis
Subjects
Details
- ISSN :
- 10111344
- Volume :
- 203
- Database :
- OpenAIRE
- Journal :
- Journal of Photochemistry and Photobiology B: Biology
- Accession number :
- edsair.doi.dedup.....2cf84a6155d94ae0de8384023dbf1248