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Host transcriptional response to TB preventive therapy differentiates two sub-groups of IGRA-positive individuals
- Source :
- bioRxiv pre, Tuberculosis (Edinburgh, Scotland)
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Supplementary data: available online at https://www.sciencedirect.com/science/article/pii/S1472979220302006?via%3Dihub#appsec1 Copyright © 2020 The Authors. We hypothesised that individuals with immunological sensitisation to Mycobacterium tuberculosis (Mtb), conventionally regarded as evidence of latent tuberculosis infection (LTBI), would demonstrate binary responses to preventive therapy (PT), reflecting the differential immunological consequences of the sterilisation of viable infection in those with active Mtb infection versus no Mtb killing in those who did not harbour viable bacilli. We investigated longitudinal whole blood transcriptional profile responses to PT of Interferon gamma release assay (IGRA)-positive tuberculosis contacts and IGRA-negative, tuberculosis-unexposed controls. Longitudinal unsupervised clustering analysis with a subset of 474 most variable genes in antigen-stimulated blood separated the IGRA-positive participants into two distinct subgroups, one of which clustered with the IGRA-negative controls. 117 probes were differentially expressed over time between the two cluster groups, many of them associated with immunological pathways important in mycobacterial control. We contend that the differential host RNA response reflects lack of Mtb viability in the group that clustered with the IGRA-negative unexposed controls, and Mtb viability in the group (1/3 of IGRA-positives) that clustered away. Gene expression patterns in the blood of IGRA-positive individuals emerging during the course of PT, which reflect Mtb viability, could have major implications in the identification of risk of progression, treatment stratification and biomarker development. This work was supported by a British Infection Association Small Project Research Grant (2016) and a Rosetrees Trust Seed Corn Award (# JS15/M660). C.B. was funded by an Academic Clinical Fellowship from the National Institute for Health Research (NIHR) (ACF-2012-18-008) and currently receives support from an Imperial 4i Wellcome Trust/NIHR Imperial BRC Clinical PhD Fellowship. M.K. receives support from the NIHR Imperial College BRC and the Wellcome Trust (Sir Henry Wellcome Fellowship grant no. 206508/Z/17/Z). J.C. receives support from the Medical Research Council Newton Fund (#MR/P017568/1).
- Subjects :
- Adult
Male
0301 basic medicine
Microbiology (medical)
Time Factors
Tuberculosis
Transcription, Genetic
latent tuberculosis infection
030106 microbiology
Immunology
Antitubercular Agents
Interferon gamma release assay
Biology
Microbiology
Article
Transcriptome
Mycobacterium tuberculosis
Young Adult
03 medical and health sciences
Latent Tuberculosis
Predictive Value of Tests
medicine
preventive therapy
Humans
Latent tuberculosis infection
Longitudinal Studies
Gene
11 Medical and Health Sciences
Whole blood
Latent tuberculosis
Gene Expression Profiling
medicine.disease
biology.organism_classification
Treatment Outcome
030104 developmental biology
Infectious Diseases
Case-Control Studies
Host-Pathogen Interactions
Biomarker (medicine)
Female
Preventive therapy
transcriptome
Immunologic Memory
Interferon-gamma Release Tests
Subjects
Details
- ISSN :
- 14729792
- Volume :
- 127
- Database :
- OpenAIRE
- Journal :
- Tuberculosis
- Accession number :
- edsair.doi.dedup.....2cf59034e2c89ef7311a543132cc34b9