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Genomic analyses of primitive, wild and cultivated citrus provide insights into asexual reproduction
- Source :
- Nature Genetics. 49:765-772
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- Qiang Xu and colleagues sequence four citrus species de novo, along with 100 accessions, including primitive, wild and cultivated citrus. Their genomic analyses associate the CitRWP gene with polyembryony and suggest that regions harboring energy- and reproduction-associated genes are probably under selection in cultivated citrus. The emergence of apomixis—the transition from sexual to asexual reproduction—is a prominent feature of modern citrus. Here we de novo sequenced and comprehensively studied the genomes of four representative citrus species. Additionally, we sequenced 100 accessions of primitive, wild and cultivated citrus. Comparative population analysis suggested that genomic regions harboring energy- and reproduction-associated genes are probably under selection in cultivated citrus. We also narrowed the genetic locus responsible for citrus polyembryony, a form of apomixis, to an 80-kb region containing 11 candidate genes. One of these, CitRWP, is expressed at higher levels in ovules of polyembryonic cultivars. We found a miniature inverted-repeat transposable element insertion in the promoter region of CitRWP that cosegregated with polyembryony. This study provides new insights into citrus apomixis and constitutes a promising resource for the mining of agriculturally important genes.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Transposable element
Citrus
Candidate gene
Population
Polyembryony
Asexual reproduction
Biology
Polymorphism, Single Nucleotide
01 natural sciences
Genome
Chromosomes, Plant
03 medical and health sciences
Species Specificity
Gene Expression Regulation, Plant
Apomixis
Reproduction, Asexual
Genetics
Cluster Analysis
education
Gene
Phylogeny
education.field_of_study
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Chromosome Mapping
Genetic Variation
food and beverages
Genomics
Sequence Analysis, DNA
Gene Ontology
030104 developmental biology
Genome, Plant
010606 plant biology & botany
Subjects
Details
- ISSN :
- 15461718 and 10614036
- Volume :
- 49
- Database :
- OpenAIRE
- Journal :
- Nature Genetics
- Accession number :
- edsair.doi.dedup.....2cc950d95837dd93e87643197d1b7bab