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A role for syndecan-1 in coupling fascin spike formation by thrombospondin-1
- Source :
- ResearcherID, The Journal of Cell Biology
-
Abstract
- An important role of cell matrix adhesion receptors is to mediate transmembrane coupling between extracellular matrix attachment, actin reorganization, and cell spreading. Thrombospondin (TSP)-1 is a modulatory component of matrix expressed during development, immune response, or wound repair. Cell adhesion to TSP-1 involves formation of biochemically distinct matrix contacts based on stable fascin spikes. The cell surface adhesion receptors required have not been identified. We report here that antibody clustering of syndecan-1 proteoglycan specifically transduces organization of cortical actin and fascin bundles in several cell types. Transfection of COS-7 cells with syndecan-1 is sufficient to stimulate cell spreading, fascin spike assembly, and extensive protrusive lateral ruffling on TSP-1 or on syndecan-1 antibody. The underlying molecular mechanism depends on glycosaminoglycan (GAG) modification of the syndecan-1 core protein at residues S45 or S47 for cell membrane spreading and on the VC2 region of the cytoplasmic domain for spreading and fascin spike formation. Expression of the VC2 deletion mutant or GAG-negative syndecan-1 showed that syndecan-1 is necessary in spreading and fascin spike formation by C2C12 cells on TSP-1. These results establish a novel role for syndecan-1 protein in coupling a physiological matrix ligand to formation of a specific matrix contact structure.
- Subjects :
- CD36 Antigens
Integrins
Cell Separation
Culture Media, Serum-Free
Syndecan 1
Cell membrane
Extracellular matrix
Thrombospondin 1
Mice
Cell-matrix adhesion
Cell Movement
Cells, Cultured
Cytoskeleton
Chelating Agents
Glycosaminoglycans
0303 health sciences
Membrane Glycoproteins
biology
030302 biochemistry & molecular biology
Microfilament Proteins
Vinculin
protrusions
Flow Cytometry
Cell biology
medicine.anatomical_structure
embryonic structures
Original Article
Proteoglycans
actin
animal structures
Syndecans
extracellular matrix
Recombinant Fusion Proteins
Integrin
Immunoblotting
CD47 Antigen
macromolecular substances
Transfection
Cell Line
03 medical and health sciences
Antigens, CD
medicine
Cell Adhesion
Animals
Humans
Cell adhesion
Edetic Acid
030304 developmental biology
Fascin
proteoglycan
Cell Membrane
Cell Biology
Actins
carbohydrates (lipids)
Microscopy, Fluorescence
biology.protein
Cell Surface Extensions
Syndecan-1
Carrier Proteins
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- ResearcherID, The Journal of Cell Biology
- Accession number :
- edsair.doi.dedup.....2cb9829ee11c31b1ed206d0fe42bbce8