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Mesenchymal cells appearing in pancreatic tissue culture are bone marrow-derived stem cells with the capacity to improve transplanted islet function
- Source :
- Stem cells (Dayton, Ohio). 28(1)
- Publication Year :
- 2009
-
Abstract
- Adherent fibroblast-like cells have been reported to appear in cultures of human endocrine or exocrine pancreatic tissue during attempts to differentiate human beta cells from pancreatic precursors. A thorough characterization of these mesenchymal cells has not yet been completed, and there are no conclusive data about their origin. We demonstrated that the human mesenchymal cells outgrowing from cultured human pancreatic endocrine or exocrine tissue are pancreatic mesenchymal stem cells (pMSC) that propagate from contaminating pMSC. The origin of pMSC is partly extrapancreatic both in humans and mice, and by using green fluorescent protein (GFP(+)) bone marrow transplantation Adherent fibroblast-like cells have been reported to appear in cultures of human endocrine or exocrine pancreatic tissue during attempts to differentiate human beta cells from pancreatic precursors. A thorough characterization of these mesenchymal cells has not yet been completed, and there are no conclusive data about their origin.We demonstrated that the human mesenchymal cells outgrowing from cultured human pancreatic endocrine or exocrine tissue are pancreatic mesenchymal stem cells (pMSC) that propagate from contaminating pMSC. The origin of pMSC is partly extrapancreatic both in humans and mice, and by using green fluorescent protein (GFP(+)) bone marrow transplantation in the mouse model, we were able to demonstrate that these cells derive from the CD45(+) component of bone marrow. The pMSC express negligible levels of islet-specific genes both in basal conditions and after serum deprivation or exogenous growth factor exposure, and might not represent optimal candidates for generation of physiologically competent beta-cells. On the other hand, when cotransplanted with a minimal pancreatic islet mass, pMSC facilitate the restoration of normoglycemia and the neovascularization of the graft. These results suggest that pMSCs could exert an indirect role of "helper" cells in tissue repair processes
- Subjects :
- Blood Glucose
Male
Time Factors
Islets of Langerhans Transplantation
Neovascularization
Mice
Cell Movement
Insulin-Secreting Cells
AC133 Antigen
Angiogenic Proteins
5'-Nucleotidase
Cells, Cultured
Stem cell transplantation for articular cartilage repair
Bone Marrow Transplantation
geography.geographical_feature_category
Gene Expression Regulation, Developmental
Amniotic stem cells
Cell Differentiation
Anatomy
Islet
Endothelial stem cell
medicine.anatomical_structure
Molecular Medicine
Stem cell
medicine.symptom
Adult stem cell
Green Fluorescent Proteins
Clinical uses of mesenchymal stem cells
Mice, Nude
Neovascularization, Physiologic
Bone Marrow Cells
Mice, Transgenic
Biology
Diabetes Mellitus, Experimental
Islets of Langerhans
Antigen
Antigens, CD
medicine
Animals
Humans
Cell Proliferation
Glycoproteins
geography
Cell growth
Mesenchymal stem cell
Endothelial Cells
Mesenchymal Stem Cells
Cell Biology
Mice, Inbred C57BL
Immunology
Cancer research
Leukocyte Common Antigens
Bone marrow
Peptides
Developmental Biology
Subjects
Details
- ISSN :
- 15494918
- Volume :
- 28
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Stem cells (Dayton, Ohio)
- Accession number :
- edsair.doi.dedup.....2cb645400b9d37c82f33dccd70e296ab