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DYF-4 regulates patched-related/DAF-6-mediated sensory compartment formation in C. elegans
- Source :
- PLoS Genetics, Vol 17, Iss 6, p e1009618 (2021), PLoS Genetics
- Publication Year :
- 2021
- Publisher :
- Public Library of Science (PLoS), 2021.
-
Abstract
- Coordination of neurite extension with surrounding glia development is critical for neuronal function, but the underlying molecular mechanisms remain poorly understood. Through a genome-wide mutagenesis screen in C. elegans, we identified dyf-4 and daf-6 as two mutants sharing similar defects in dendrite extension. DAF-6 encodes a glia-specific patched-related membrane protein that plays vital roles in glial morphogenesis. We cloned dyf-4 and found that DYF-4 encodes a glia-secreted protein. Further investigations revealed that DYF-4 interacts with DAF-6 and functions in a same pathway as DAF-6 to regulate sensory compartment formation. Furthermore, we demonstrated that reported glial suppressors of daf-6 could also restore dendrite elongation and ciliogenesis in both dyf-4 and daf-6 mutants. Collectively, our data reveal that DYF-4 is a regulator for DAF-6 which promotes the proper formation of the glial channel and indirectly affects neurite extension and ciliogenesis.<br />Author summary In C. elegans sensory organ, the ciliated neuronal endings are wrapped in a luminal channel formed by glial cells, forming a specialized sensory compartment critical for sensory activity. Coordination of glial channel formation, dendritic tip anchoring and ciliogenesis are critical for the formation of a functional sensory compartment. DAF-6, a patched-related glial membrane protein, was reported to play an important role in glial channel morphogenesis, but the molecular function and regulatory mechanism of DAF-6 remain poorly understood. Here, we found that DYF-4, a glia-secreted protein, interacts and colocalizes with DAF-6, and functions in a same pathway as DAF-6 to regulate sensory compartment formation. We propose that DYF-4 is a novel regulator for DAF-6 to control sensory compartment formation.
- Subjects :
- Cancer Research
Nematoda
Regulator
Gene Expression
Social Sciences
Cell Communication
QH426-470
0302 clinical medicine
Animal Cells
Morphogenesis
Medicine and Health Sciences
Psychology
Cloning, Molecular
Genetics (clinical)
Neurons
0303 health sciences
Cilium
Intracellular Signaling Peptides and Proteins
Gene Expression Regulation, Developmental
Eukaryota
Animal Models
Recombinant Proteins
Cell biology
Phenotypes
Experimental Organism Systems
Caenorhabditis Elegans
Sensory Perception
Dendrite extension
Cellular Types
Cellular Structures and Organelles
Anatomy
Neuroglia
Research Article
Patched
Neurogenesis
Genetic Vectors
Nerve Tissue Proteins
Nose
Biology
Research and Analysis Methods
03 medical and health sciences
Model Organisms
Ciliogenesis
Escherichia coli
Neurites
Genetics
Animals
Compartment (development)
Cilia
Caenorhabditis elegans Proteins
Molecular Biology
Ecology, Evolution, Behavior and Systematics
030304 developmental biology
Genome, Helminth
fungi
Cognitive Psychology
Organisms
Biology and Life Sciences
Cell Biology
Neuronal Dendrites
Invertebrates
Membrane protein
Mutagenesis
Cellular Neuroscience
Face
Animal Studies
Caenorhabditis
Cognitive Science
Perception
Sensory Neurons
Zoology
Head
030217 neurology & neurosurgery
Neuroscience
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 15537404 and 15537390
- Volume :
- 17
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- PLoS Genetics
- Accession number :
- edsair.doi.dedup.....2cb5d4a88a2930e0a46397f8b273d601