Supplemental Figure 4 from Herbacetin Is a Novel Allosteric Inhibitor of Ornithine Decarboxylase with Antitumor Activity

The effect of herbacetin on cell cycle. The effect of herbacetin or DFMO on cell cycle was determined using HCT116, DLD1 or HT29 colon cancer cells. Cells were treated with herbacetin or DFMO for 48 h in medium supplemented with 10% FBS and then cells were serum starved for 24 h in medium supplemented with 0.5% FBS. Cells were stained with propidium iodide (PI) and cell cycle was analyzed by Fluorescence Activated Cell Sorting (FACS). All data are represented as mean values {plus minus} S.D. of triplicate values from 3 independent experiments. The asterisk (*) indicates a significant effect (p < 0.05) of herbacetin or DFMO compared to untreated control.