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Antineoplastic effect of iodine in mammary cancer: participation of 6-iodolactone (6-IL) and peroxisome proliferator-activated receptors (PPAR)
- Source :
- Molecular Cancer, Molecular Cancer, Vol 8, Iss 1, p 33 (2009)
- Publication Year :
- 2009
-
Abstract
- Introduction Studies in mammary cancer demonstrated that moderately high concentrations of molecular iodine (I2) have a antiproliferative and apoptotic effect either in vivo as in vitro, however the cellular intermediated involved in these effects has not been elucidated. Methods Virgin Sprague-Dawley rats were treated with methyl-nitrosourea (MNU: single dose ip, 50 mg/Kg bw) and the participation of arachidonic acid (AA) and PPAR receptors in the antineoplasic effect of I2 where analyzed. Results I2-treated rats for four weeks exhibited a significant reduction in the incidence (62.5 vs. 100%) and size (0.87 ± 0.98 vs 1.96 ± 1.5 cm3) of mammary tumors. HPLC analysis showed that tumoral but not normal mammary tissue contained an elevated basal concentration of AA and significantly more AA-iodinated called 6-iodolactone (6-IL) after chronic I2 treatment. Tumors from I2-treated rats showed fewer cells positive to proliferating cell nuclear antigen, lower blood vessel density, as well as decreases in vascular endothelial growth factor, urokinase-type plasminogen activator, and PPAR type alpha (PPARα). These same tumors showed increases in the cell death markers, TUNEL-positive cells (p < 0.05) and the enzyme caspase-3 (trend), as well as significant induction of PPAR type gamma (PPARγ). Conclusion Together, these data demonstrate that the antineoplasic effect of iodine involves 6-IL formation and PPARγ induction.
- Subjects :
- Vascular Endothelial Growth Factor A
Cancer Research
medicine.medical_specialty
Peroxisome Proliferator-Activated Receptors
Peroxisome proliferator-activated receptor
Antineoplastic Agents
Apoptosis
Arachidonic Acids
Biology
lcsh:RC254-282
Rats, Sprague-Dawley
chemistry.chemical_compound
Internal medicine
medicine
Animals
Receptor
Chromatography, High Pressure Liquid
Cell Proliferation
chemistry.chemical_classification
Analysis of Variance
Arachidonic Acid
Cell growth
Caspase 3
Research
Mammary Neoplasms, Experimental
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Immunohistochemistry
Urokinase-Type Plasminogen Activator
Rats
Vascular endothelial growth factor
Vascular endothelial growth factor A
Endocrinology
Oncology
chemistry
Molecular Medicine
Arachidonic acid
Female
Plasminogen activator
Iodine
Subjects
Details
- ISSN :
- 14764598
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Molecular cancer
- Accession number :
- edsair.doi.dedup.....2c66982b9df5d4890b65c0411f5217dd