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Defect in messenger RNA for human hemoglobin synthesis in beta thalassemia
- Source :
- Journal of Clinical Investigation. 50:2755-2760
- Publication Year :
- 1971
- Publisher :
- American Society for Clinical Investigation, 1971.
-
Abstract
- Functional messenger RNA for human hemoglobin synthesis was prepared from reticulocyte lysates of patients with homozygous beta thalassemia and sickle cell anemia. The messenger RNA stimulated the synthesis of human globin chains by a cell-free system derived from Krebs mouse ascites cells. In the presence of beta thalassemia messenger RNA, the system synthesized much less beta chain than alpha chain whereas in the presence of sickle cell anemia messenger RNA, nearly equal amounts of alpha and beta chains were synthesized. The beta/alpha synthetic ratios obtained in the cell-free system were similar to those obtained by incubating intact beta thalassemia and sickle cell anemia reticulocytes in the presence of radioactive leucine. The experiments provide direct evidence of a defect in messenger RNA for beta chains as a cause for the decreased synthesis of beta chains observed in beta thalassemia.
- Subjects :
- Sucrose
Reticulocytes
Thalassemia
Centrifugation
Anemia, Sickle Cell
Biology
Hemoglobins
Mice
Reticulocyte
hemic and lymphatic diseases
Methods
medicine
Animals
Chemical Precipitation
Humans
RNA, Messenger
Globin
Beta (finance)
Cells, Cultured
Messenger RNA
Cell-Free System
Beta thalassemia
RNA
Articles
General Medicine
Electrophoresis, Disc
medicine.disease
Molecular biology
medicine.anatomical_structure
Biochemistry
Spectrophotometry
Rabbits
Alpha chain
Subjects
Details
- ISSN :
- 00219738
- Volume :
- 50
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Investigation
- Accession number :
- edsair.doi.dedup.....2c6171f6abc6e4c046f05e4496760acd