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1-Methoxylespeflorin G11 Protects HT22 Cells from Glutamate-Induced Cell Death through Inhibition of ROS Production and Apoptosis
- Source :
- Journal of Microbiology and Biotechnology. 31:217-225
- Publication Year :
- 2021
- Publisher :
- Journal of Microbiology and Biotechnology, 2021.
-
Abstract
- This study aimed to investigate the neuroprotective effects of 1-methoxylespeflorin G11 (MLG), a pterocarpan, against glutamate-induced neurotoxicity in neuronal HT22 hippocampal cells. The protective effects of MLG were evaluated using MTT assay and microscopic analysis. The extent of apoptosis was studied using flow cytometric analysis performed on the damaged cells probed with annexin V/propidium iodide. Moreover, mitochondrial reactive oxygen species (ROS) were assessed using flow cytometry through MitoSOXTM Red staining. To determine mitochondrial membrane potential, staining with tetramethylrhodamine and JC-1 was performed followed by flow cytometry. The results demonstrated that MLG attenuates glutamate-induced apoptosis in HT22 cells by inhibiting intracellular ROS generation and mitochondrial dysfunction. Additionally, MLG prevented glutamate-induced apoptotic pathway in HT22 cells through upregulation of Bcl-2 and downregulation of cleaved PARP-1, AIF, and phosphorylated MAPK cascades. In addition, MLG treatment induced HO-1 expression in HT22 cells. These results suggested that MLG exhibits neuroprotective effects against glutamate-induced neurotoxicity in neuronal HT22 cells by inhibiting oxidative stress and apoptosis.
- Subjects :
- 0106 biological sciences
Programmed cell death
Pterocarpans
Poly (ADP-Ribose) Polymerase-1
Glutamic Acid
Apoptosis
medicine.disease_cause
01 natural sciences
Applied Microbiology and Biotechnology
Cell Line
Flow cytometry
Mice
chemistry.chemical_compound
Annexin
010608 biotechnology
medicine
Animals
MTT assay
Propidium iodide
Neurons
chemistry.chemical_classification
Reactive oxygen species
Cell Death
medicine.diagnostic_test
Chemistry
Membrane Proteins
General Medicine
Mitochondria
Cell biology
Oxidative Stress
Neuroprotective Agents
Reactive Oxygen Species
Heme Oxygenase-1
Oxidative stress
Biotechnology
Subjects
Details
- ISSN :
- 17388872 and 10177825
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Journal of Microbiology and Biotechnology
- Accession number :
- edsair.doi.dedup.....2c55dd17fb35868fa0ad195639c1c443