Back to Search
Start Over
Carboxyl-terminal sequence of the glycine-arginine-rich histone from bovine lymphosarcoma, Novikoff hepatoma and fetal calf thymus
- Source :
- Biochimica et biophysica acta. 181(1)
- Publication Year :
- 1969
-
Abstract
- 1. 1. The glycine-arginine-rich histones isolated from bovine lymphosarcoma and Novikoff hepatoma have been found to have virtually the same amino acid compositions as that of fetal cal thymus and calf thymus published previously 8,9 . 2. 2. The glycine-arginine-rich histones isolated from fetal calf thymus, bovine lymphosarcoma and Novikoff hepatoma were cleaved with cyanogen bromide to yield two peptides. The C-terminal CN-2 peptide for each histone was isolated by the use of Sephadex G-100. The C-terminal CN-2 peptide for each of these three tissues was found to have the same amino acid composition as that from calf thymus 12 . 3. 3. The C-terminal peptides, CN-2, of bovine lymphosarcoma, fetal calf thymus and Novikoff hepatoma were hydrolyzed with trypsin. In each case five peptides were obtained by peptide mapping and ion-exchange chromatography. The amino acid compositions of these peptides were identical for each of the tissues and to that found for calf thymus previously. 4. 4. It was concluded that the C-terminal portion of the glycine-arginine-rich histones isolaetd from these four tissues are identical with each other and with that of pea seedling 11 .
- Subjects :
- Electrophoresis
Carcinoma, Hepatocellular
Chemical Phenomena
Glycine
Peptide
Thymus Gland
Arginine
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Histones
Hydrolysis
chemistry.chemical_compound
Species Specificity
medicine
Animals
Trypsin
Amino Acids
chemistry.chemical_classification
Fetus
Cyanides
biology
Lymphoma, Non-Hodgkin
Liver Neoplasms
Bromine
Chromatography, Ion Exchange
digestive system diseases
Amino acid
Chemistry
Histone
Biochemistry
chemistry
Sephadex
biology.protein
Chromatography, Gel
Cyanogen bromide
Cattle
Peptides
medicine.drug
Subjects
Details
- ISSN :
- 00063002
- Volume :
- 181
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Biochimica et biophysica acta
- Accession number :
- edsair.doi.dedup.....2c273a773f3f6f77312b59cfbb2043c9