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STUDIES OF DNA-INDUCED HERITABLE ALTERATION OF MAMMALIAN CELLS
- Source :
- The Journal of Experimental Medicine
- Publication Year :
- 1970
- Publisher :
- The Rockefeller University Press, 1970.
-
Abstract
- An intercellular interaction between mouse Ehrlich ascites tumor and non-malignant Chinese hamster cells occurred when these were co-cultured. That the intercellular processes which formed had emanated from the EA cells was revealed by immunofluoroscopy using anti-EA antiserum, and by direct microscopic examination. A passage of DNA from the EA to the CH cells was also observed. On long-term co-culture, new cell forms arose which were isolated, cloned, and propagated. They showed a CH karyotype and had acquired oncogenic potential and the ability to synthesize murine-specific antigens. These same heritable properties were also acquired by CH cells following their exposure to DNA isolated from EA cells.
- Subjects :
- Immunodiffusion
Immunology
Cell
Fluorescent Antibody Technique
Chinese hamster
Article
Cell Line
chemistry.chemical_compound
Mice
Antigen
Cricetinae
Culture Techniques
medicine
Immunology and Allergy
Animals
Carcinoma, Ehrlich Tumor
Molecular Biology
Antiserum
biology
Karyotype
DNA
biology.organism_classification
Molecular biology
medicine.anatomical_structure
Cell Transformation, Neoplastic
chemistry
Cell culture
Karyotyping
Autoradiography
Intracellular
Subjects
Details
- Language :
- English
- ISSN :
- 15409538 and 00221007
- Volume :
- 132
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- The Journal of Experimental Medicine
- Accession number :
- edsair.doi.dedup.....2c2573c464a927987538fc6da7328d28