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Aptamer−Nanoparticle Strip Biosensor for Sensitive Detection of Cancer Cells
- Source :
- Analytical Chemistry. 81:10013-10018
- Publication Year :
- 2009
- Publisher :
- American Chemical Society (ACS), 2009.
-
Abstract
- We report an aptamer-nanoparticle strip biosensor (ANSB) for the rapid, specific, sensitive and low-cost detection of circulating cancer cells. Known for their high specificity and affinity, aptamers were first selected from live cells by the cell-SELEX (systematic evolution of ligands by exponential enrichment) process. When next combined with the unique optical properties of gold nanoparticles (Au-NPs), ANSBs were prepared on a lateral flow device. Ramos cells were used as a model target cell to demonstrate proof of principle. Under optimal conditions, the ANSB was capable of detecting a minimum of 4000 Ramos cells without instrumentation (visual judgment) and 800 Ramos cells with a portable strip reader within 15 minutes. Importantly, ANSB has successfully detected Ramos cells in human blood, thus providing a rapid, sensitive and low-cost quantitative tool for the detection of circulating cancer cells. ANSB therefore shows great promise for in-field and point-of-care cancer diagnosis and therapy.
- Subjects :
- Chemistry
Aptamer
Cell
Metal Nanoparticles
Nanoparticle
Nanotechnology
Biosensing Techniques
Cell Separation
Aptamers, Nucleotide
Ligands
Neoplastic Cells, Circulating
Sensitivity and Specificity
Article
Analytical Chemistry
medicine.anatomical_structure
Cell culture
Colloidal gold
Cell Line, Tumor
Cancer cell
Biophysics
medicine
Humans
Gold
Biosensor
Systematic evolution of ligands by exponential enrichment
Subjects
Details
- ISSN :
- 15206882 and 00032700
- Volume :
- 81
- Database :
- OpenAIRE
- Journal :
- Analytical Chemistry
- Accession number :
- edsair.doi.dedup.....2c1bdeb560cc4c8d3bdc9f6a18f6a941