Back to Search
Start Over
Multi-component identification and target cell-based screening of potential bioactive compounds in toad venom by UPLC coupled with high-resolution LTQ-Orbitrap MS and high-sensitivity Qtrap MS
- Source :
- Analytical and bioanalytical chemistry. 410(18)
- Publication Year :
- 2018
-
Abstract
- Traditional Chinese medicines (TCMs) are undoubtedly treasured natural resources for discovering effective medicines in treating and preventing various diseases. However, it is still extremely difficult for screening the bioactive compounds due to the tremendous constituents in TCMs. In this work, the chemical composition of toad venom was comprehensively analyzed using ultra-high performance liquid chromatography (UPLC) coupled with high-resolution LTQ-Orbitrap mass spectrometry and 93 compounds were detected. Among them, 17 constituents were confirmed by standard substances and 8 constituents were detected in toad venom for the first time. Further, a compound database of toad venom containing the fullest compounds was further constructed using UPLC coupled with high-sensitivity Qtrap MS. Then a target cell-based approach for screening potential bioactive compounds from toad venom was developed by analyzing the target cell extracts. The reliability of this method was validated by negative controls and positive controls. In total, 17 components in toad venom were discovered to interact with the target cancer cells. Further, in vitro pharmacological trials were performed to confirm the anti-cancer activity of four of them. The results showed that the six bufogenins and seven bufotoxins detected in our research represented a promising resource to explore bufogenins/bufotoxins-based anticancer agents with low cardiotoxic effect. The target cell-based screening method coupled with the compound database of toad venom constructed by UPLC-Qtrap-MS with high sensitivity provide us a new strategy to rapidly screen and identify the potential bioactive constituents with low content in natural products, which was beneficial for drug discovery from other TCMs. ᅟ Graphical abstract.
- Subjects :
- 0301 basic medicine
High resolution
Antineoplastic Agents
01 natural sciences
Biochemistry
High-performance liquid chromatography
Mass Spectrometry
Analytical Chemistry
Toad Venom
03 medical and health sciences
Limit of Detection
Drug Discovery
Screening method
Animals
Humans
Chromatography, High Pressure Liquid
Biological Products
Chromatography
Chemistry
Drug discovery
010401 analytical chemistry
Orbitrap ms
Reproducibility of Results
Hep G2 Cells
Bufonidae
0104 chemical sciences
030104 developmental biology
Amphibian Venoms
MCF-7 Cells
Drug Screening Assays, Antitumor
Cell based
Subjects
Details
- ISSN :
- 16182650
- Volume :
- 410
- Issue :
- 18
- Database :
- OpenAIRE
- Journal :
- Analytical and bioanalytical chemistry
- Accession number :
- edsair.doi.dedup.....2c126fb9c5b4ea7cee77d8f1cd940d90