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Eucalyptol protects lungs against bacterial invasion through attenuating ciliated cell damage and suppressing MUC5AC expression
- Source :
- Journal of cellular physiology. 234(5)
- Publication Year :
- 2017
-
Abstract
- This study was conducted to investigate whether eucalyptol plays a role in influencing bacterial growth in cigarette smoke-exposed lungs. Rats were exposed to air (control) and cigarette smoke (smoking) in the presence and absence of eucalyptol (260 mg/day). Morphological analysis of lung structures and status of airway mucous production were observed under microscope. Pathological changes of ciliated columnar epithelium in airways were examined using transmission electron microscopy. MUC5AC protein and messenger RNA (mRNA) expression in bronchoalveolar lavage fluid (BALF) and lungs were determined. Application of eucalyptol reduced pulmonary bullae formation and airway mucus overproduction in the smoke-exposed lungs. Treatment with eucalyptol attenuated ciliated cell damage in cigarette smoke-exposed lungs. Bacterial colonies of lungs were obviously lower in the eucalyptol-treated rats than that in the smoking rats (p < 0.01). Treatment with eucalyptol reduced the counts of bacterial colonization residing in the challenged lungs (p < 0.01). Application of eucalyptol not only decreased MUC5AC protein expression in BALF and tobacco-exposed lungs but also suppressed its mRNA expression in the lungs (all p < 0.05). Intervention of eucalyptol benefits elimination of bacterial organisms from tobacco-exposed lungs through attenuating ciliated cell damage and suppressing MUC5AC expression in the lungs.
- Subjects :
- 0301 basic medicine
Male
Physiology
Clinical Biochemistry
Down-Regulation
Bacterial growth
Mucin 5AC
Microbiology
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
Pulmonary Disease, Chronic Obstructive
0302 clinical medicine
Smoke
medicine
Animals
Cilia
Lung
Messenger RNA
Eucalyptol
medicine.diagnostic_test
Bacteria
Chemistry
Ciliated columnar epithelium
Epithelial Cells
Cell Biology
Tobacco Products
respiratory system
Mucus
Bacterial Load
respiratory tract diseases
Anti-Bacterial Agents
Disease Models, Animal
030104 developmental biology
Bronchoalveolar lavage
medicine.anatomical_structure
030228 respiratory system
Host-Pathogen Interactions
Airway
Subjects
Details
- ISSN :
- 10974652
- Volume :
- 234
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Journal of cellular physiology
- Accession number :
- edsair.doi.dedup.....2c10f7714a69319e71e4a5d750aa100e