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Lead induces oxidative stress, DNA damage and alteration of p53, Bax and Bcl-2 expressions in mice
- Source :
- Food and Chemical Toxicology. 46:1488-1494
- Publication Year :
- 2008
- Publisher :
- Elsevier BV, 2008.
-
Abstract
- Oxidative stress is considered as a possible molecular mechanism involved in lead toxicity. This study was carried out to investigate whether lead acetate could induce oxidative stress in mice, and the following damages as well. Lead acetate was given orally to mice for 4 weeks at doses of 0, 10, 50, 100mg/kg body weight every other day, respectively. Production of reactive oxygen species (ROS) and malondialdehyde (MDA) were measured as indicators of oxidative stress. DNA damage in peripheral blood lymphocytes was determined by comet assay. Ultrastructure alteration was detected using transmission electron microscopy. The alterations of p53, Bax, and Bcl-2 expression were determined by western blotting. The results showed that lead acetate significantly increased the levels of ROS and MDA in mice. Meanwhile, severe DNA damage and ultrastructure alterations were obviously observed. In addition, p53 and Bax expressions increased and the imbalance of Bax/Bcl-2 occurred. Therefore, it strongly suggests that lead may induce oxidative stress and change the expressions of apoptosis-related proteins in mouse liver.
- Subjects :
- Male
DNA damage
Blotting, Western
Biology
Toxicology
medicine.disease_cause
Lipid peroxidation
Mice
chemistry.chemical_compound
Bcl-2-associated X protein
Malondialdehyde
medicine
Animals
bcl-2-Associated X Protein
chemistry.chemical_classification
Mice, Inbred ICR
Reactive oxygen species
Glyceraldehyde-3-Phosphate Dehydrogenases
General Medicine
Molecular biology
Comet assay
Oxidative Stress
Lead
Liver
Proto-Oncogene Proteins c-bcl-2
chemistry
Biochemistry
Lead acetate
biology.protein
Comet Assay
Lipid Peroxidation
Tumor Suppressor Protein p53
Reactive Oxygen Species
Oxidative stress
DNA Damage
Food Science
Subjects
Details
- ISSN :
- 02786915
- Volume :
- 46
- Database :
- OpenAIRE
- Journal :
- Food and Chemical Toxicology
- Accession number :
- edsair.doi.dedup.....2bf892791b78c09c8136e96f88287ad6