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Effects of Standardized Green Tea Extract and Its Main Component, EGCG, on Mitochondrial Function and Contractile Performance of Healthy Rat Cardiomyocytes
- Source :
- Nutrients, Nutrients, Vol 12, Iss 2949, p 2949 (2020), Volume 12, Issue 10
- Publication Year :
- 2020
- Publisher :
- MDPI, 2020.
-
Abstract
- We recently showed that the long-term in vivo administration of green tea catechin extract (GTE) resulted in hyperdynamic cardiomyocyte contractility. The present study investigates the mechanisms underlying GTE action in comparison to its major component, epigallocatechin-3-gallate (EGCG), given at the equivalent amount that would be in the entirety of GTE. Twenty-six male Wistar rats were given 40 mL/day of a tap water solution with either standardized GTE or pure EGCG for 4 weeks. Cardiomyocytes were then isolated for the study. Cellular bioenergetics was found to be significantly improved in both GTE- and EGCG-fed rats compared to that in controls as shown by measuring the maximal mitochondrial respiration rate and the cellular ATP level. Notably, the improvement of mitochondrial function was associated with increased levels of oxidative phosphorylation complexes, whereas the cellular mitochondrial mass was unchanged. However, only the GTE supplement improved cardiomyocyte mechanics and intracellular calcium dynamics, by lowering the expression of total phospholamban (PLB), which led to an increase of both the phosphorylated-PLB/PLB and the sarco-endoplasmic reticulum calcium ATPase/PLB ratios. Our findings suggest that GTE might be a valuable adjuvant tool for counteracting the occurrence and/or the progression of cardiomyopathies in which mitochondrial dysfunction and alteration of intracellular calcium dynamics constitute early pathogenic factors.
- Subjects :
- 0301 basic medicine
Male
oxidative phosphorylation
green tea extracts
lcsh:TX341-641
Oxidative phosphorylation
Green tea extract
Mitochondrion
Pharmacology
Calcium in biology
Article
Catechin
Sarcoplasmic Reticulum Calcium-Transporting ATPases
Contractility
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Animals
Myocytes, Cardiac
Rats, Wistar
phospholamban
Nutrition and Dietetics
Cardiomyocyte mechanic
Tea
Chemistry
Plant Extracts
cardiomyocyte mechanics
Calcium-Binding Proteins
Phospholamban
Mitochondria
Rats
Calcium ATPase
SERCA2
030104 developmental biology
030220 oncology & carcinogenesis
Energy Metabolism
lcsh:Nutrition. Foods and food supply
Food Science
Subjects
Details
- Language :
- English
- ISSN :
- 20726643
- Volume :
- 12
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Nutrients
- Accession number :
- edsair.doi.dedup.....2bcf2791dfd4e72efacbd5390dccf9e3