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Identification and modelling of fast and slow I h current components in vestibular ganglion neurons
- Source :
- The European Journal of Neuroscience, European Journal of Neuroscience, European Journal of Neuroscience, Wiley, 2015, 42 (10), pp.2867-2877. ⟨10.1111/ejn.13021⟩, European Journal of Neuroscience, 2015, 42 (10), pp.2867-2877. ⟨10.1111/ejn.13021⟩
- Publication Year :
- 2015
- Publisher :
- John Wiley and Sons Inc., 2015.
-
Abstract
- International audience; Previous experimental data indicates the hyperpolarization-activated cation (Ih ) current, in the inner ear, consists of two components [different hyperpolarization-activated cyclic nucleotide-gated (HCN) subunits] which are impossible to pharmacologically isolate. To confirm the presence of these two components in vestibular ganglion neurons we have applied a parameter identification algorithm which is able to discriminate the parameters of the two components from experimental data. Using simulated data we have shown that this algorithm is able to identify the parameters of two populations of non-inactivated ionic channels more accurately than a classical method. Moreover, the algorithm was demonstrated to be insensitive to the key parameter variations. We then applied this algorithm to Ih current recordings from mouse vestibular ganglion neurons. The algorithm revealed the presence of a high-voltage-activated slow component and a low-voltage-activated fast component. Finally, the electrophysiological significance of these two Ih components was tested individually in computational vestibular ganglion neuron models (sustained and transient), in the control case and in the presence of cAMP, an intracellular cyclic nucleotide that modulates HCN channel activity. The results suggest that, first, the fast and slow components modulate differently the action potential excitability and the excitatory postsynaptic potentials in both sustained and transient vestibular neurons and, second, the fast and slow components, in the control case, provide different information about characteristics of the stimulation and this information is significantly modified after modulation by cAMP.
- Subjects :
- Male
Voltage clamp
computational modelling
Models, Neurological
Scarpa's ganglion
Action Potentials
hyperpolarization-activated cation current
Biology
Vestibular Nerve
Cyclic nucleotide
chemistry.chemical_compound
[SPI]Engineering Sciences [physics]
Mice
Ganglia, Sensory
medicine
Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
Animals
Inner ear
Computer Simulation
voltage clamp
Vestibular system
Computational Neuroscience
Neurons
vestibular
General Neuroscience
Electrophysiology
medicine.anatomical_structure
chemistry
Excitatory postsynaptic potential
Female
Neuron
hyperpolarization‐activated cation current
Neuroscience
Algorithms
Subjects
Details
- Language :
- English
- ISSN :
- 14609568 and 0953816X
- Volume :
- 42
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- The European Journal of Neuroscience
- Accession number :
- edsair.doi.dedup.....2bbe7d14fa85511fc14442e04e80d096